Flow Cytometry Antibody Panel for One-Step Clonal Disease Screening
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Solution Overview
Problem
Current flow cytometry methods for diagnosing clonal diseases are complex, prone to misdiagnosis, and lack standardization, making them difficult to automate and inefficient for detecting rare tumors and pre-leukemic lesions.
Innovation Solution
A comprehensive antibody combination for flow cytometry, comprising eight groups of antibodies, allows for one-step screening and diagnosis of clonal diseases, including malignancies, PNH, AA, and pre-leukemic lesions, using a 5-tube parallel protocol with specific antibody combinations and gating strategies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional flow cytometry methods are used for diagnosing clonal diseases, then detection capability is provided, but the methods are complex, prone to misdiagnosis, and lack standardization
Solution Approach 1:
The detection method is segmented into distinct functional modules: a first detection panel for initial screening of clonal diseases and a second detection panel for targeted analysis of specific disease types. This segmentation reduces overall complexity by allowing systematic approach rather than attempting to detect all diseases simultaneously with a single complex panel.
Solution Approach 2:
The invention creates a universal detection system where the first detection panel can screen for multiple types of clonal diseases (leukemia, lymphoma, myeloma, PNH, aplastic anemia) using a standardized protocol. This multi-functional approach eliminates the need for multiple specialized panels, reducing complexity while maintaining comprehensive detection capability.
2Productivity
If traditional flow cytometry methods are used, then disease detection is possible, but automation is difficult and efficiency is low
Solution Approach 1:
The invention standardizes detection parameters including fixed antibody concentrations, standardized flow cytometry acquisition settings, and defined analysis criteria. These standardized parameters enable automated execution of the detection protocol, as the system no longer requires manual adjustment of parameters for different disease types, significantly improving both automation capability and detection efficiency.
3Adaptability or versatility
If comprehensive screening for all clonal diseases is attempted, then detection coverage is improved, but misdiagnosis increases due to lack of standardization
Solution Approach 1:
The detection system is segmented into two sequential panels: the first panel performs comprehensive screening for all clonal diseases using standardized criteria, while the second panel provides targeted analysis for specific disease types identified in the first panel. This segmentation maintains comprehensive coverage while improving accuracy by avoiding the confusion of attempting to detect all diseases simultaneously with a single non-standardized panel.
Solution Approach 2:
The first detection panel performs preliminary screening for all clonal diseases using standardized acquisition and analysis criteria before the second panel is activated for targeted analysis. This preliminary action ensures that all disease types are identified systematically and accurately before specialized analysis, preventing misdiagnosis while maintaining comprehensive coverage.
Data Source
AI summary
Disclosed are an antibody combination for one-step screening and/or diagnosis of clonal diseases and application thereof. The antibody combination includes eight groups of antibodies, and is a set of flow cytometry detection panels for one-step screening and/or diagnosis of clonal diseases, and 5-tube parallel is used for one sample, the first group of antibodies and the second group of antibodies are used for samples in different flow cytometry tubes, the third group of antibodies and the sixth group of antibodies are used for samples in the same flow cytometry tube, the fourth group of antibodies and the seventh group of antibodies are used for samples in the same flow cytometry tube, and the fifth group of antibodies and the eighth group of antibodies are used for samples in the same flow cytometry tube.