Flow Chemistry Glycan Hydrolysis and NADA Derivatization

The patent implements continuous flow chemistry where glycan hydrolysis and saccharide derivatization occur continuously in a flow reactor system rather than in discrete batch steps. The reactants flow continuously through the system, allowing uninterrupted reaction and eliminating the need for manual transfer and setup between batches, thereby significantly improving productivity and reducing total process time.

The patent combines multiple reaction steps (hydrolysis and derivatization) into a single integrated flow chemistry system. The flow reactor simultaneously performs acid-catalyzed hydrolysis of glycans and subsequent derivatization of released monosaccharides with NADA, merging what were traditionally separate batch operations into one continuous process, thus enhancing efficiency and reducing time loss.

The patent optimizes reaction parameters in the flow chemistry system, including temperature, flow rate, and reactant concentrations, to maximize the formation of the desired NAIM derivative while minimizing isomeric byproducts. By precisely controlling these parameters in the continuous flow system, the reaction yield is improved while maintaining the high detection sensitivity provided by the NADA label.

The patent uses the flow chemistry system itself as an intermediary that facilitates the derivatization reaction. The controlled environment of the flow reactor allows for optimal mixing and reaction conditions, enabling efficient conversion of monosaccharides to NAIM derivatives despite the inherent isomeric challenges, thus improving both yield and detection sensitivity.