Fluid Device Oily Sealing Liquid Suppresses Noise Luminescence
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing fluid device technologies face challenges in achieving high detection accuracy for small amounts of target substances due to noise luminescence interference, which degrades detection precision.
Innovation Solution
A fluid device with a substrate and lid structure that includes an inlet, outlet, and annular wall, using an oily sealing liquid with higher density than water to isolate and seal reaction spaces within micro-wells, reducing noise interference and enhancing detection accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If luminescence is used for detection reaction in a fluid device with multiple wells, then detection capability is achieved, but noise luminescence mixes with signal luminescence and detection accuracy decreases
Solution Approach 1:
The invention divides the detection system into spatially separated independent wells, where each well contains a single reaction mixture. This segmentation prevents noise luminescence from one well from interfering with the detection signal in another well, thereby improving detection accuracy for small amounts of target substance.
Solution Approach 2:
The invention introduces an oily sealing liquid as an intermediary substance that forms a distinct layer between the aqueous reaction mixture and the detection system components. This sealing liquid acts as a barrier that isolates the luminescence signal from noise sources, enabling accurate detection even in the presence of noise luminescence.
2Measurement precision
If an aqueous medium is used for detection reaction, then target substance can be dissolved and reacted, but noise luminescence from the aqueous environment interferes with detection
Solution Approach 1:
The invention introduces an oily sealing liquid as an intermediary layer that separates the aqueous reaction medium from the detection environment. This sealing liquid absorbs or blocks noise luminescence from the aqueous medium, preventing it from reaching the detection system while allowing the aqueous reaction to proceed normally.
Solution Approach 2:
The invention creates different physical and chemical environments in different regions: the well interior maintains aqueous conditions for target substance dissolution and reaction, while the sealing liquid layer provides a luminescence-blocking environment at the detection interface, achieving local optimization of both reaction and detection conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The described fluid device and detection kit effectively suppress noise luminescence, improving detection accuracy and enabling high-precision detection of target substances by creating isolated reaction spaces within the micro-wells.
Implementation Method 1
an oily sealing liquid with a higher density than water
Implementation Method 2
sealing the aqueous medium in the plurality of wells with the oily sealing liquid
Implementation Method 3
a reaction solution is heated by heating the fluid device
Data Source
AI summary
A fluid device includes a substrate having a plurality of wells on one surface; and a first lid disposed to face the well of the substrate, in which the first lid has an inlet that penetrates the first lid in a thickness direction, an injection tube that communicates with the inlet and extends above the inlet, an outlet that penetrates the first lid in a thickness direction, a discharge tube that communicates with the outlet and extends above the outlet, and an annular wall portion in a plan view provided along a peripheral edge portion of an upper surface of the first lid, and an upper end of the wall portion is higher than upper ends of the injection tube and the discharge tube.