Multiplexing Fluid Sample Reuse Device
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Solution Overview
Problem
Current methods for detecting analytes in fluid samples require multiple samples and result in fluid sample loss or reduction, limiting the ability to detect multiple analytes from a small volume effectively.
Innovation Solution
A device comprising multiple pipettes with immobilized capture antibodies allows for the detection of multiple analytes in a single fluid sample by sequential contact and re-dispensing, enabling the reuse of the sample and minimizing loss.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple fluid samples or aliquots are used for analyte detection, then detection sensitivity and reliability are improved, but fluid sample volume requirement increases and sample loss increases
Solution Approach 1:
The device divides the single fluid sample into multiple sequential portions, each contacted with different capture antibodies in separate pipettes. This segmentation allows multiple analytes to be detected from one sample without requiring multiple initial samples or large aliquots, thereby reducing overall sample loss while maintaining detection reliability.
Solution Approach 2:
The device recovers and re-uses the fluid sample by sequentially contacting it with multiple capture antibodies across different pipettes. Instead of discarding the sample after a single detection event, the system recovers it and continues to use it for detecting additional analytes, thereby minimizing sample loss while improving detection reliability through multiplexing.
2Adaptability or versatility
If multiple aliquots are taken from a single fluid sample, then multiple analytes can be detected, but the volume of the single fluid sample required increases
Solution Approach 1:
The fluid sample serves multiple functions by being sequentially contacted with different capture antibodies in different pipettes. A single small-volume sample can detect multiple analytes across multiple detection panels, making the sample universally applicable for various detection purposes without requiring large volumes or multiple aliquots.
Solution Approach 2:
The device adds a temporal dimension to sample utilization by sequentially contacting the sample with different capture antibodies across multiple pipettes rather than requiring simultaneous parallel processing of multiple aliquots. This dimensional approach allows multiplexed detection from a single small-volume sample over time.
3Loss of substance
If sequential contact with multiple capture antibodies is implemented, then fluid sample reuse is enabled and sample loss is minimized, but device complexity increases
Solution Approach 1:
Multiple pipettes containing different capture antibodies are nested within a single device housing with a common sample reservoir. The sample is sequentially transferred through nested pipette structures, enabling fluid sample reuse across multiple detection events while containing the complexity within a unified device architecture rather than requiring separate standalone systems.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the detection of multiple analytes in a small volume of fluid sample without significant loss, facilitating a multiplexed panel analysis.
Implementation Method 1
the first immobilized binding partner is brought into contact with the withdrawn fluid sample to thereby form a first analyte-binding partner complex if a first analyte is present in the fluid sample
Data Source
AI summary
Devices, methods, and kits for detecting at least two analytes present within a small volume single fluid sample obtained from patient for the creation of a multiplexed panel of the various analytes present within the single fluid sample are disclosed.