Fluidic Device Holder Alignment for Precise Flow Cell Imaging
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Solution Overview
Problem
Existing systems for biological or chemical analysis, such as sequencing-by-synthesis protocols, are limited in capability and cost-effective, necessitating improved systems and methods for performing assays in a simpler and more efficient manner.
Innovation Solution
A fluidic device with a floatable flow cell and gasket alignment, a removable cartridge for positioning, and a device holder with alignment members, along with an optical assembly for precise alignment and imaging, is provided to enhance the efficiency and cost-effectiveness of sample analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If existing systems for biological or chemical analysis are used, then the analysis can be performed, but the system capability is limited and cost-effectiveness is reduced
Solution Approach 1:
The system is divided into separate functional modules: a removable cartridge containing the flow cell, a device holder with alignment members, and a gasket system. This segmentation allows each component to be optimized independently while maintaining overall system capability, and enables cost-effective manufacturing through standardized, interchangeable parts
Solution Approach 2:
The device holder with alignment members and the removable cartridge design create a universal platform that can accommodate different flow cells and assay configurations. This multi-functionality enhances system adaptability while maintaining cost-effectiveness through a standardized interface system
2Measurement precision
If precise alignment and imaging are required for sample analysis, then analysis accuracy is improved, but system complexity increases
Solution Approach 1:
Alignment members are pre-installed on the device holder, and the gasket is pre-configured with inlet/outlet passages positioned to align with flow cell ports. This preliminary preparation ensures precise alignment when components are assembled, achieving high measurement precision without requiring complex real-time adjustment mechanisms
Solution Approach 2:
The gasket acts as an intermediary component between the flow cell and the housing. It provides both sealing and alignment functions, with its pre-configured inlet/outlet passages serving as a mediator to ensure proper positioning of the flow cell relative to the housing features, thereby achieving precise alignment without direct mechanical constraints
3Ease of operation
If a floatable flow cell design is used, then ease of insertion and removal is improved, but positioning stability may be compromised
Solution Approach 1:
The floatable flow cell design allows the flow cell to self-position within the housing through buoyancy forces when inserted. The inlet and outlet ports automatically align with the gasket passages without requiring manual adjustment, providing both ease of operation and reliable positioning through the system's own physical properties rather than external intervention
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables precise alignment and imaging of samples, facilitating automated and cost-effective biological or chemical assays, including sequencing protocols, by improving the alignment and imaging processes.
Implementation Method 1
The gasket is coupled to the housing. The gasket has inlet and outlet passages and comprises a compressible material.
Implementation Method 2
The reception space is sized and shaped to permit the flow cell to float relative to the housing.
Data Source
AI summary
A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.