Fluidic Device Holder Alignment for Precise Optical Sample Imaging
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Solution Overview
Problem
Existing systems for biological or chemical analysis, such as sequencing-by-synthesis protocols, are limited in capability and cost-effective, necessitating improved systems for performing assays in a simpler and more efficient manner.
Innovation Solution
A fluidic device with a floatable flow cell and gasket alignment, a removable cartridge for positioning, and a fluidic device holder that orients samples with respect to perpendicular axes, combined with an optical assembly and imaging system for precise detection of optical signals.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If existing systems for biological or chemical analysis are used, then the assays can be performed, but the systems are limited in capability and not cost-effective
Solution Approach 1:
The system is divided into modular components including a flow cell, cartridge, fluidic device holder, and optical assembly. Each module can be independently manufactured, assembled, and replaced, enabling cost-effective production while maintaining advanced capabilities. The flow cell separates the sample analysis function from the housing and imaging system.
Solution Approach 2:
The fluidic device holder and cartridge design provide multi-functional capabilities, allowing the same basic structure to accommodate different flow cells and sample types. The system can perform various biological and chemical assays using a unified platform, improving cost-effectiveness while expanding adaptability.
2Measurement precision
If precise alignment and imaging of samples is implemented, then detection accuracy is improved, but system complexity increases
Solution Approach 1:
The floatable flow cell design allows the flow cell to float and self-align with the optical assembly at a predetermined position. This eliminates the need for complex mechanical alignment mechanisms while achieving precise positioning for optimal optical detection. The gasket structure provides a predetermined position that ensures proper alignment between the flow cell and imaging system.
Solution Approach 2:
The flow cell automatically positions itself through floating and alignment with the gasket structure, without requiring external alignment mechanisms. The system self-adjusts to achieve the predetermined position for optimal imaging, reducing mechanical complexity while maintaining detection precision.
3Productivity
If automated detection of optical signals is implemented, then productivity is improved, but device complexity increases
Solution Approach 1:
The optical assembly is integrated with the fluidic device holder and cartridge structure, combining the imaging function with the sample handling system. This merged design enables automated detection without requiring separate complex positioning and control systems, as the optical path is built into the modular structure.
Solution Approach 2:
The gasket structure serves as an intermediary component that not only seals the flow cell but also provides alignment features and structural support for the optical assembly. This multi-functional intermediary element reduces the need for additional alignment mechanisms while enabling automated imaging.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances the capability and cost-effectiveness of biological or chemical assays by allowing precise alignment and imaging of samples, facilitating automated and efficient detection of optical signals from desired reactions.
Implementation Method 1
The optical assembly is configured to direct optical signals through the flow cell to the detector
Implementation Method 2
These chemical reactions may be observed by exciting the labels with radiation and detecting light emissions from the labels
Implementation Method 3
After nucleotides are added to the sstDNAs clusters, an image in four channels is taken (i.e., one for each fluorescent label)
Data Source
AI summary
A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.