Fluorescence Observation Device Dynamic Binning Control
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Solution Overview
Problem
Fluorescence observation devices face challenges in achieving optimal image quality and sensitivity, particularly in low-fluorescence conditions, where existing methods rely on increasing pixel binning or exposure time, which may not adequately address image quality and sensitivity needs.
Innovation Solution
A fluorescence observation device that includes an excitation light source, an imaging element for acquiring fluorescence images, an image quality evaluating section to calculate SN ratio from luminance information, and a sensitivity adjusting section to adjust pixel binning or exposure time based on calculated SN ratio, ensuring the ratio meets or exceeds a predetermined threshold.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the number of pixels for binning summing is increased or exposure time is lengthened, then sensitivity for observation is improved, but image quality and resolution deteriorate
Solution Approach 1:
The patent implements dynamic adjustment of binning summing parameters and exposure time based on real-time SN ratio calculations. The sensitivity adjusting section modifies the number of pixels for binning summing and/or exposure time according to calculated SN ratios, enabling the system to adapt sensitivity settings to actual imaging conditions rather than using fixed parameters
Solution Approach 2:
The patent establishes a feedback loop where the image quality evaluating section calculates SN ratios from acquired fluorescence images, and this information feeds back to the sensitivity adjusting section which then modifies binning summing and exposure time settings. This closed-loop control ensures that sensitivity adjustments are based on actual image quality metrics
Solution Approach 3:
The patent changes operational parameters (number of pixels for binning summing and exposure time) based on calculated SN ratios. The sensitivity adjusting section modifies these parameters dynamically to maintain optimal image quality while ensuring sufficient sensitivity for detecting fluorescence signals
2Measurement precision
If exposure time is increased to detect weak fluorescence, then sensitivity is improved, but image blur increases
Solution Approach 1:
The system dynamically adjusts exposure time based on real-time SN ratio calculations rather than using a fixed exposure time. The sensitivity adjusting section modifies exposure time according to actual imaging conditions, allowing the system to use longer exposure times only when necessary while maintaining shorter exposure times when sufficient signal is present, thereby reducing overall image blur
Solution Approach 2:
The feedback mechanism uses SN ratio calculations from acquired images to determine appropriate exposure time settings. The image quality evaluating section provides feedback on image quality, and the sensitivity adjusting section adjusts exposure time accordingly, creating a closed-loop control that prevents excessive exposure time and resulting image blur
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution enables optimal fluorescence observation by dynamically adjusting sensitivity settings to maintain minimum image quality while minimizing image blur, ensuring accurate and effective fluorescence imaging even in low-fluorescence conditions.
Implementation Method 1
a fluorescence image acquiring section provided with an imaging element that acquires a fluorescence image by imaging fluorescence produced in the subject when the excitation light emitted from the excitation light source is radiated onto the subject
Data Source
AI summary
Observation is performed at a more appropriate sensitivity without reducing an image quality. Provided is a fluorescence observation device (1) including: an excitation light source (3) that emits excitation light to be radiated onto a subject (A); a fluorescence-image acquiring section (21) provided with an imaging element (18) that acquires a fluorescence image (G2) by imaging fluorescence produced in the subject (A) when the excitation light emitted from the excitation light source (3) is radiated onto the subject (A); and a sensitivity adjusting section (22) that adjusts, based on luminance information of the fluorescence image (G2) acquired by the imaging element (18) of the fluorescence-image acquiring section (21), a number of pixels for binning summing (B) and/or an exposure time (t) in the imaging element (18) such that a SN ratio of the fluorescence image (G2) is equal to or larger than a predetermined threshold.