Fluorescence Imaging Brightness Control via Light Intensity Adjustment
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Solution Overview
Problem
In fluorescence observation, maintaining constant brightness while avoiding white balance skew in background images is challenging due to mixed reflected excitation light, as existing methods prioritize control parameters like light intensity and gain, leading to noise increase and reduced image quality.
Innovation Solution
A processing apparatus that adjusts the intensity and sensitivity of both white light and excitation light sources based on obtained image information to maintain a predetermined ratio, ensuring consistent brightness and minimizing white balance variations in background images.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If gain is increased to adjust brightness of fluorescent image, then brightness is improved, but noise component is increased and image quality is reduced
Solution Approach 1:
The patent changes the control parameter from gain to excitation light intensity. By adjusting the intensity of the excitation light source instead of increasing gain, the system can improve fluorescent image brightness without introducing the noise amplification problem that occurs when gain is increased. This parameter substitution resolves the contradiction between brightness improvement and image quality maintenance.
2Illumination intensity
If brightness of fluorescent image is adjusted via gain without changing excitation light intensity, then brightness is improved, but white balance of background image is skewed
Solution Approach 1:
The patent substitutes gain adjustment with excitation light intensity adjustment as the control parameter. When excitation light intensity is changed, the fluorescent image brightness changes accordingly, while the background image (illuminated by white light) maintains its original white balance characteristics. This parameter substitution eliminates the white balance skewing problem that occurs when gain is adjusted independently.
Solution Approach 2:
The patent separates the control of fluorescent image brightness from background image white balance by using different control parameters: excitation light intensity for fluorescent images and white light intensity for background images. This segmentation allows independent optimization of each image type without mutual interference, resolving the contradiction between brightness adjustment and white balance stability.
3Illumination intensity
If white light intensity is decreased to adjust background image brightness, then brightness is improved, but white balance of background image becomes skewed
Solution Approach 1:
The patent introduces excitation light intensity as an alternative control parameter for background image brightness adjustment. Instead of decreasing white light intensity (which skews white balance), the system adjusts excitation light intensity to achieve the desired brightness change while maintaining the background image's white balance through separate white light control.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Automatically adjusts brightness in both background and fluorescent images, maintaining image quality by controlling light sources' intensity and sensitivity, thereby suppressing white balance variations and noise.
Implementation Method 1
a first obtaining unit configured to obtain first image information relating to brightness adjustment of a first image obtained from light including light of a first light source
Implementation Method 2
when observing fluorescence (fluorescence observation) produced by emitting X-rays, ultraviolet light, infrared light, or visible light as an excitation light
Data Source
AI summary
A processing apparatus includes: a first obtaining unit configured to obtain first image information relating to brightness adjustment of a first image obtained from light including light of a first light source and light of a second light source different from the first light source; a second obtaining unit configured to obtain second image information relating to brightness adjustment of a second image obtained from light of the second light source; and a control unit configured to control each of intensity of a first irradiation light emitted by the first light source and sensitivity of the first image and intensity of a second irradiation light emitted by the second light source and sensitivity of the second image.