Fluorescence Observation Apparatus Crosstalk Correction
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Solution Overview
Problem
Current fluorescence observation techniques face challenges in accurately distinguishing and separating fluorescent light emissions from multiple fluorescent probes due to crosstalk phenomena, which affect the accuracy of cancer diagnosis and staging.
Innovation Solution
A fluorescence observation apparatus that includes a light source for exciting both fluorescent substances, a detection system for capturing their emissions, a correction value calculation section to account for color mixing, and an image correction section to adjust luminance values based on spectral characteristics, thereby reducing crosstalk and enhancing image clarity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple fluorescent probes are used for cancer diagnosis, then diagnostic information and staging accuracy are improved, but crosstalk between fluorescent lights degrades image contrast and measurement precision
Solution Approach 1:
The patent segments the fluorescent light detection by wavelength bands, separating the detection of first fluorescent light (e.g., 500-600nm) and second fluorescent light (e.g., 600-700nm) using wavelength-selective filters or spectrally-resolved detectors. This segmentation allows independent measurement of each fluorescent probe's emission, eliminating crosstalk and enabling accurate quantification of multiple probes simultaneously for improved cancer diagnosis.
Solution Approach 2:
The patent introduces correction values as an intermediary computational element that mediates between the raw mixed fluorescent signals and the final diagnostic measurements. These correction values, derived from spectral characteristics and concentration relationships, mathematically compensate for crosstalk effects, allowing accurate reconstruction of individual fluorescent probe signals even when spectral overlap occurs.
2Measurement precision
If fluorescent light intensity is increased to improve signal detection, then detection sensitivity is improved, but color mixture between different fluorescent lights increases
Solution Approach 1:
The patent employs feedback through correction value calculation, where the system continuously measures the actual fluorescent light intensities, compares them against expected spectral characteristics, and dynamically adjusts correction values to compensate for color mixture. This feedback loop enables the system to maintain high detection sensitivity while preserving spectral information by mathematically separating overlapping fluorescent signals based on their known spectral profiles.
Solution Approach 2:
The patent changes the parameter representation of fluorescent light from simple intensity values to spectral parameter sets that include wavelength distribution and intensity ratios. By representing each fluorescent probe's emission in terms of its spectral fingerprint rather than single-wavelength intensity, the system can distinguish between different probes even at high intensities, preventing information loss due to color mixture.
3Illumination intensity
If probe concentration is increased to enhance fluorescent signal, then signal intensity is improved, but crosstalk and color mixture effects are amplified
Solution Approach 1:
The patent performs preliminary action by pre-calculating correction values based on the known spectral characteristics and concentration relationships of the fluorescent probes before actual diagnostic measurement. These pre-computed correction factors account for expected crosstalk at various concentration levels, allowing the system to compensate for color mixture effects even when probe concentrations are high, thereby maintaining accurate multi-probe detection capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus effectively reduces crosstalk-induced degradation of contrast, allowing for stable and accurate observation of fluorescent light emissions from multiple probes without relying on probe concentration or excitation light intensity, improving cancer diagnosis and staging accuracy.
Implementation Method 1
a light source section for emitting excitation light to be applied to a first fluorescent substance and a second fluorescent substance; a fluorescence detection section for detecting first fluorescent light emitted when the first fluorescent substance is excited by the excitation light and second fluorescent light emitted when the second fluorescent substance is excited by the excitation light
Data Source
AI summary
A fluorescence observation apparatus according to the present invention includes a light source section that emits excitation light for exciting a first fluorescent substance and a second fluorescent substance, a fluorescence detection section that detects first fluorescent light emitted when the first fluorescent substance is excited and second fluorescent light emitted when the second fluorescent substance is excited, a correction value calculation section that calculates a correction value for canceling a color mixture between the first fluorescent light and the second fluorescent light based on feature values corresponding to characteristics of the first fluorescent substance and the second fluorescent substance, a detected image generation section that generates a first detected image corresponding to a detection result of the first fluorescent light and a second detected image corresponding to a detection result of the second fluorescent light, and an image correction section that corrects a luminance value of the first detected image to a luminance value corresponding to intensity of the first fluorescent light and corrects a luminance value of the second detected image to a luminance value corresponding to intensity of the second fluorescent light.