Fluorescence Data Correction for Real-Time PCR
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Solution Overview
Problem
Existing methods for data correction in fluorescence data, particularly in real-time PCR, are not accurate or flexible, and often integrated within measurement systems, limiting their standalone use and flexibility in detecting target molecules and infections.
Innovation Solution
A computer-implemented method for data correction that includes time shift, colour compensation, subtraction of background signals, and moving average processing, allowing for standalone operation and integration with measurement equipment, with customizable options for data formats and calibration parameters to enhance detection of target molecules and infections.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If data correction methods are integrated within measurement systems, then ease of operation is improved, but flexibility and accuracy are worsened
Solution Approach 1:
The patent segments the data correction process into distinct modular components that can be independently selected and applied. The system separates the measurement function from the correction function, allowing correction algorithms to be independently optimized and chosen based on specific application requirements rather than being fixed within the measurement device.
Solution Approach 2:
The patent introduces an intermediary data processing layer between the measurement system and the final analysis. This intermediary layer provides standardized data interfaces while allowing flexible application of various correction methods, enabling the system to maintain ease of operation while achieving high flexibility through the intermediary correction module.
2Measurement precision
If multiple correction steps are applied to fluorescence data, then measurement precision is improved, but device complexity is worsened
Solution Approach 1:
The correction process is divided into distinct sequential steps: background signal subtraction, time shift correction, and color compensation. Each step addresses a specific source of error independently, allowing the system to achieve high measurement precision while maintaining manageable complexity through modular processing stages.
Solution Approach 2:
The patent applies corrections in a predetermined sequence where preliminary steps (background subtraction) prepare the data for subsequent corrections (time shift, color compensation). This preliminary action approach ensures that each correction step builds on properly prepared data, maximizing precision while avoiding the complexity of simultaneous multi-parameter optimization.
3Measurement precision
If time shift correction is applied to fluorescence measurements, then measurement precision is improved, but loss of time is worsened
Solution Approach 1:
The patent applies time shift correction selectively based on the specific measurement conditions and requirements. Rather than universally applying complex time correction algorithms to all data, the system determines when correction is necessary and applies appropriate levels of correction, reducing unnecessary processing time while maintaining precision where needed.
Data Source
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AI summary
The invention concerns (computer implemented) methods for data correction, in particular, fluorescence data, related systems, software, graphic user interfaces and the use thereof, more in particular, the invention describes a method for adjusting a part of measurements taken from a plurality of measurements, under the same time-dependent area, at different time points in which said adjustments are performed in order to obtain calculated measurements which can be compared as if they were taken at the same time point (and hence, under the same environment).