Fluorescence Detection Position Adjustment for Chip Variance
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Solution Overview
Problem
Existing fluorescence detecting apparatuses face accuracy issues due to individual differences among analysis chips and apparatuses caused by manufacturing errors, leading to variations in the irradiation position of the excitation light beam and subsequent fluorescence intensity, which deteriorates analysis accuracy.
Innovation Solution
A fluorescence detecting apparatus and method that includes light irradiating, fluorescence detecting, and irradiation position adjusting means to determine the optimal irradiation position of the excitation light beam based on adjustment fluorescence signals, ensuring consistent light beam irradiation across different analysis chips by shifting and scanning the light beam to compensate for positional fluctuations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If analysis chips are produced with predetermined sizes, then manufacturing is simplified, but dimensional shifts due to manufacturing errors cause irradiation position variations and deteriorate analysis accuracy
Solution Approach 1:
The system performs preliminary measurement of the actual irradiation position on each analysis chip before formal analysis. By measuring fluorescence intensity at multiple positions and determining the optimal position in advance, the system compensates for manufacturing dimensional shifts and ensures accurate irradiation positioning for subsequent analysis.
Solution Approach 2:
The system changes the irradiation position parameter dynamically based on measured fluorescence intensity values. By adjusting the irradiation position to the optimal location determined through preliminary measurements, the system compensates for chip dimensional variations and maintains consistent analysis accuracy across different chips.
2Adaptability or versatility
If individual analysis chips are used for different samples, then sample-specific analysis is enabled, but individual differences among chips due to manufacturing errors lead to variations in detected fluorescence intensity
Solution Approach 1:
Before performing sample-specific analysis on each analysis chip, the system performs a preliminary measurement to determine the optimal irradiation position for that specific chip. This preliminary action enables the system to adapt to individual chip characteristics while maintaining consistent fluorescence detection across different chips and samples.
Solution Approach 2:
Each analysis chip essentially adjusts its own irradiation position based on its specific characteristics measured during the preliminary phase. The system allows each chip to self-optimize its irradiation position according to its individual manufacturing variations, enabling consistent results across diverse chips.
3Device complexity
If the irradiation position is fixed for all chips, then the apparatus structure is simplified, but manufacturing errors cause variations in irradiation position and deteriorate analysis accuracy
Solution Approach 1:
The system transitions from a fixed irradiation position to a dynamic, adjustable irradiation position that is optimized for each analysis chip. By making the irradiation position variable and adapting it based on preliminary measurements, the system maintains analysis accuracy without requiring complex hardware modifications.
Solution Approach 2:
The system uses feedback from preliminary fluorescence intensity measurements to determine and adjust the optimal irradiation position. This feedback mechanism allows the apparatus to automatically compensate for manufacturing variations and maintain high analysis accuracy while keeping the overall structure relatively simple.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables accurate analysis by automatically adjusting the irradiation position of the excitation light beam, maintaining consistent analysis conditions across various analysis chips, thereby preventing deterioration in analysis accuracy due to individual differences.
Implementation Method 1
an analysis chip having a test region, for capturing a detection target substance included in a sample solution, within a flow channel
Implementation Method 2
irradiates an excitation light beam onto the test region, and detects fluorescence generated at the test region
Data Source
AI summary
Excitation light beam is irradiated onto a test region while shifting the irradiation position thereof, when performing analysis of a target substance using an analysis chip having the test region within a flow channel through which a sample solution is caused to flow, for capturing the target substance within the sample solution. Fluorescence generated at the test region is detected as a plurality of adjustment fluorescence signals. The target substance is analyzed using fluorescence generated when the excitation light beam is irradiated at an irradiation position within the test region determined based on the plurality of adjustment fluorescence signals.