In-liquid Fluorescence Detection Device Raman Interference Suppression
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Solution Overview
Problem
In-liquid fluorescence detection is hindered by Raman scattering light interference, which overlaps with the fluorescence emitted by particles in liquids, making it difficult to accurately differentiate between biotic and abiotic particles due to autofluorescence and Raman scattering light interference.
Innovation Solution
An in-liquid fluorescence detection device that emits excitation light with a wavelength positioned between the fluorescence wavelength bands of biotic and abiotic particles, using separate photodetectors to detect fluorescence in specific wavelength bands while excluding Raman scattering light, allowing for accurate differentiation based on intensity ratios.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a liquid is irradiated with excitation light to detect fluorescent particles, then fluorescence detection is enabled, but Raman scattering light interferes with the detection accuracy
Solution Approach 1:
The detection device segments the fluorescence spectrum into multiple wavelength bands (first, second, and third bands) and detects each band separately using dedicated photodetectors. This segmentation allows the system to distinguish fluorescence signals from Raman scattering light by analyzing the spectral distribution across different bands, thereby resolving the interference problem while maintaining detection accuracy.
Solution Approach 2:
The invention transitions from single-wavelength detection to multi-wavelength band detection, adding a spectral dimension to the measurement. By detecting fluorescence intensity across multiple wavelength bands and comparing the ratios between them, the system can differentiate between fluorescence and Raman scattering light, effectively eliminating the interference while preserving measurement precision.
2Measurement precision
If excitation light wavelength is chosen to detect one type of fluorescent particle, then detection sensitivity for that particle type is improved, but detection capability for other particle types with different fluorescence wavelengths is reduced
Solution Approach 1:
The detection device is designed with multiple photodetectors that can simultaneously detect fluorescence across multiple wavelength bands. This multi-functional configuration enables the system to detect different types of fluorescent particles (such as microorganisms and abiotic particles) with different characteristic wavelengths using the same device, achieving universal detection capability while maintaining sensitivity for each particle type through spectral ratio analysis.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device effectively suppresses Raman scattering light interference, enabling accurate detection of autofluorescence and distinguishing between microorganisms and abiotic particles by analyzing intensity differences in specific fluorescence wavelength bands.
Implementation Method 1
a light source emitting excitation light having a wavelength in which Raman scattering light is generated in a liquid in a wavelength band between first and second fluorescence wavelength bands toward the liquid
Implementation Method 2
Raman scattering light with a level of intensity that interferes with detection of fluorescent particles emitting autofluorescence is not generated when a gas is irradiated with an excitation light, however, when a liquid is irradiated with an excitation light, Raman scattering light with the level of intensity that interferes with detection of fluorescent particles emitting autofluorescence may be generated
Data Source
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AI summary
[Problem] To provide an in-liquid fluorescence detection device capable of accurately detecting fluorescence in a liquid. [Means for Resolution] A in-liquid fluorescence detection device including a light source 10 emitting an excitation light having a wavelength in which Raman scattering light is generated in a liquid in a wavelength band between first and second fluorescence wavelength bands toward the liquid which can contain a first substance which emits light having higher intensities in the first fluorescence wavelength band as compared with in the second fluorescence wavelength band and a second substance which emits light having higher intensities in the second fluorescence wavelength band as compared with in the first fluorescence wavelength band and a fluorescence detection unit 102 detecting light in the first and second fluorescence wavelength bands.