Fluorescence Detection Device Rough Surface Layer Signal Collection
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Solution Overview
Problem
Current fluorescence detection technologies face challenges due to the weakness of fluorescence signals, requiring additional amplification techniques that are often expensive and time-consuming, and existing supports for enhancing signals are complex and costly to use.
Innovation Solution
A fluorescence detection device with a support having a rough surface layer that redirects fluorescence in multiple directions, allowing for enhanced signal collection without prior amplification, featuring a conducting layer with specific roughness parameters and a transparent spacing layer, enabling broad aperture detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If additional amplification techniques are used to enhance fluorescence signal, then signal strength is improved, but cost, time, and complexity increase
Solution Approach 1:
The support structure itself performs the signal enhancement function through its metallic layer and rough surface geometry, eliminating the need for external amplification techniques. The system uses the support's physical structure to redirect and concentrate fluorescence light, making the detection device self-sufficient without requiring additional amplification equipment or procedures.
Solution Approach 2:
The patent replaces complex mechanical/amplification systems with an optical solution based on light redirection. Instead of using amplification techniques that may involve multiple steps and components, the invention uses a metallic layer with specific roughness to optically redirect fluorescence photons toward the detector, simplifying the overall system.
2Measurement precision
If specific observation techniques with evanescent waves are used, then fluorescence detection sensitivity is improved, but device complexity and usage configuration requirements increase
Solution Approach 1:
The support structure with its metallic layer and rough surface serves multiple functions simultaneously: it enhances fluorescence signal, redirects light in multiple directions, and works with standard detection configurations. This universal design eliminates the need for specific observation techniques like evanescent waves, making the device easier to operate while maintaining high sensitivity.
Solution Approach 2:
The rough surface geometry redirects fluorescence light from a limited directional range into a broader angular distribution. By utilizing the spatial dimension of light propagation and the geometric features of the rough surface, the system expands the collection angle without requiring complex optical configurations, thereby simplifying operation while enhancing detection sensitivity.
3Illumination intensity
If mirror type supports with transparent spacing layers are used, then fluorescence signal enhancement is achieved, but device complexity and cost increase
Solution Approach 1:
Instead of using a perfect mirror with uniform properties, the invention employs a metallic layer with controlled roughness that provides localized variations in light redirection. This local quality approach, where the rough surface features are distributed throughout the metallic layer, achieves signal enhancement without requiring the complex precision of perfect mirrors, thereby reducing device complexity while maintaining effectiveness.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enhances fluorescence signal collection efficiency, simplifying detection and reducing the need for complex or expensive amplification methods, while allowing for precise measurement of sample thickness and inter-fluorophore energy transfers.
Implementation Method 1
a layer having a rough surface for redirecting said fluorescence in a plurality of directions
Implementation Method 2
Some supports as those described in U.S. Pat. No. 5,866,433 are based on enhanced fluorescence induced by the presence of metallic nanoparticles under a transparent and inert separating layer
Data Source
AI summary
This invention relates to a fluorescence detection device comprising a support means (101) for supporting a sample (111), a sample excitation means so that a fluorescence is emitted by the sample (111) and a detection means for detecting said fluorescence, said support means (101) comprising a layer having a rough surface (115) for redirecting said fluorescence in a plurality of directions and said detection means covering an observation cone to collect the redirected fluorescence in a plurality of directions.