Fluorescence Detection Wavelength Selection
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Solution Overview
Problem
Conventional fluorescence detection methods face challenges in accurately determining the concentration of samples with unknown concentrations due to distortion of detection waveforms and differences in detection sensitivity between standard and inspection samples, leading to complex operation procedures and high costs when automating the detection of multiple samples with varying concentrations.
Innovation Solution
The method involves irradiating a sample with an exciting light ray and detecting fluorescent luminescence rays across multiple wavelengths, selecting wavelengths that parameterize fluorescence intensities within a detection range, and outputting these intensities as detection results, allowing for simultaneous detection of samples with different concentrations without the need for concentration adjustment, using a system comprising a light source, detector, memory unit, and program for selecting appropriate wavelengths.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If the sensitivity of an amplifier is changed to detect samples with unknown concentrations, then the detection range can be extended, but the detection waveform becomes distorted and detection sensitivity differs between standard and inspection samples
Solution Approach 1:
The patent changes the detection parameter from amplifier sensitivity to excitation light intensity. By varying the excitation light intensity across multiple levels, the system can detect samples with unknown concentrations without altering amplifier sensitivity, thus avoiding waveform distortion and maintaining consistent detection sensitivity between standard and inspection samples.
2Measurement precision
If concentration adjustment operations are performed for samples with unknown concentrations, then accurate detection within the detection range is achieved, but the operation complexity and processing time increase
Solution Approach 1:
The patent performs preliminary detection at multiple excitation light intensities before final concentration determination. By capturing fluorescence intensity data at various excitation levels in advance, the system can accurately determine sample concentration without requiring subsequent concentration adjustment operations, thereby simplifying the overall操作流程.
3Measurement precision
If multiple samples with different concentrations are detected using conventional methods, then each sample can be accurately measured, but separate concentration adjustment operations are required for each sample
Solution Approach 1:
The patent creates a universal detection method that works for samples with any concentration using the same excitation light intensities. By detecting fluorescence intensities at multiple predefined excitation levels, the system can simultaneously handle samples with different concentrations without requiring sample-specific concentration adjustment operations, thereby improving throughput.
4Extent of automation
If automated pipetting devices with concentration adjustment mechanisms are provided, then concentration adjustment can be automated, but the device complexity, cost, and scale increase
Solution Approach 1:
The patent replaces the mechanical concentration adjustment system with an optical control system. Instead of using automated pipetting devices to physically adjust sample concentrations, the system uses software-controlled variation of excitation light intensities to achieve the same effect, thereby eliminating complex mechanical components while maintaining automation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach simplifies concentration adjustment, enables accurate detection of fluorescence intensities across a wide range of concentrations, improves signal-to-noise ratio, and reduces the complexity and cost of automated fluorescence detection systems by eliminating the need for separate concentration adjustment mechanisms.
Implementation Method 1
a sample labeled with a fluorescent material is irradiated with an exciting light ray of an excitation wavelength optimized for the labeled fluorescent material and a fluorescent luminescence ray associated with a wavelength
Data Source
AI summary
In fluorescence detection, complicated concentration adjustment and retry of detection operation can be eliminated. In a fluorescence detection method for irradiating an exciting light ray on a fluorescent material or a sample having the fluorescent material to detect fluorescent luminescent rays emanated under the irradiation, the fluorescent luminescence rays parameterized by a plurality of wavelengths in a wavelength band of a fluorescent area are detected simultaneously, wavelengths which parameterize fluorescence intensities detected within a detection range are adopted from the plurality of wavelengths and the fluorescence intensities parameterized by the adopted wavelengths are delivered as detection results. The present invention also discloses a fluorescence detection apparatus and a program for a computer.