Water-Soluble Compound Enhancing Fluorescence Intensity
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Solution Overview
Problem
Existing fluorescence imaging techniques face a challenge in maintaining fluorescence intensity due to self-association of organic fluorescent dyes, leading to a decrease in brightness, which current methods to suppress self-association are not universally applicable.
Innovation Solution
Employing a water-soluble compound with a melting point of 50°C or higher, such as mannitol, sucrose, or trehalose, to act on fluorescently labeled biological substances to suppress dye interactions and enhance fluorescence intensity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If the number of organic fluorescent dye molecules per biomolecule is increased to enhance fluorescence intensity, then the desired brightness level is achieved, but self-association between dyes occurs leading to further decrease in fluorescence intensity
Solution Approach 1:
A water-soluble compound with a melting point of 50°C or higher is introduced as an intermediary substance between fluorescent dye molecules. This compound prevents direct interaction and self-association between dyes, thereby maintaining high fluorescence intensity even when multiple dye molecules are present per biomolecule.
Solution Approach 2:
The invention changes the physical parameter (melting point) of the added compound to be 50°C or higher, which correlates with its ability to effectively suppress dye self-association. This parameter threshold identifies compounds capable of maintaining structural integrity at relevant temperatures while preventing dye aggregation.
2Illumination intensity
If a fluorescent dye-labeled biomolecule is used to achieve desired fluorescence brightness, then the target substance can be detected, but self-association occurs leading to decrease in fluorescence intensity after labeling
Solution Approach 1:
The water-soluble compound acts as a protective intermediary that stabilizes the spatial arrangement of fluorescent dye molecules on the biomolecule. By preventing self-association, it ensures consistent and reliable fluorescence intensity throughout the detection process.
Solution Approach 2:
The water-soluble compound is added to the system before or during the fluorescence detection process to preemptively prevent self-association between dye molecules, thereby maintaining stable fluorescence intensity from the outset.
3Adaptability or versatility
If organic fluorescent dyes with aromatic chromophores are used to achieve fluorescence imaging, then the desired imaging capability is obtained, but self-association easily occurs due to high planarity of the chromophores
Solution Approach 1:
The water-soluble compound serves as a physical barrier between planar aromatic chromophores, preventing their tendency to stack and self-associate through pi-pi interactions, thereby maintaining imaging capability without the harmful aggregation effect.
Solution Approach 2:
The water-soluble compound creates an inert-like environment around the fluorescent dyes by forming a solvation shell or physical barrier, isolating the dyes from each other and preventing self-association while allowing the dyes to maintain their fluorescence imaging function.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of these compounds effectively enhances fluorescence intensity by interposing between dye molecules, preventing quenching and maintaining brightness, applicable across various fluorescence imaging applications.
Implementation Method 1
fluorescence intensity enhancing agent... fluorescence intensity of fluorescently labeled target biological material
Data Source
AI summary
A fluorescence intensity enhancer containing a water-soluble compound having a melting point of 50°C or higher, a fluorescence intensity enhancing method of a fluorescently labeled target biological substance using the fluorescence intensity enhancer, and a kit for fluorescence detection containing the fluorescence intensity enhancer.