Fluorescence Fading Correction for Microscopy Image Analysis
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Solution Overview
Problem
Existing fluorescence microscopy technologies fail to accurately account for fluorescence fading, leading to inaccurate evaluation of fluorescent molecules and antibodies due to the intensity of excitation rays and exposure time, which affects the apparent quantum yield and noise in captured images.
Innovation Solution
An information processing apparatus and method that corrects luminance in captured image information using a fluorescence fading coefficient, allowing for precise calculation of fluorescent molecules and antibodies by accounting for the rate of fluorescence intensity drop in the fluorescent dye reagent.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the imaging device is exposed to the excitation ray for a long time period to capture fluorescence images, then the fluorescence signal can be detected, but noise is likely to be included in the captured image information
Solution Approach 1:
The patent applies preliminary action by measuring the fluorescence intensity at multiple time points during the exposure period and using this temporal information to calculate a correction value. This correction value is then applied to the captured image to compensate for fluorescence fading, effectively separating the signal degradation from noise while maintaining measurement precision without requiring extended exposure times.
2Illumination intensity
If the intensity of the excitation ray is increased to improve fluorescence signal strength, then the fluorescence intensity increases, but fluorescence fading occurs more rapidly
Solution Approach 1:
The patent implements feedback by continuously monitoring the fluorescence intensity over time and using this information to dynamically calculate a correction value. The correction value is derived from the rate of fluorescence fading observed during the exposure period and is applied to compensate for the intensity loss, allowing the system to maintain accurate fluorescence quantification even when high excitation intensity causes rapid fading.
3Quantity of substance
If the exposure time is extended to capture sufficient fluorescence signal, then the fluorescence can be detected, but the apparent quantum yield decreases due to fluorescence fading
Solution Approach 1:
The patent applies parameter changes by calculating a correction value based on the temporal profile of fluorescence fading and applying this correction to the captured image data. This correction adjusts the measured fluorescence intensity to what would have been observed without fading, thereby restoring the apparent quantum yield to its true value while maintaining the ability to detect sufficient signal quantity through extended exposure.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate quantitative evaluation of fluorescent molecules and antibodies by correcting for fluorescence fading, improving image quality and data optimization, and allowing for comparison across different fluorescent substances.
Implementation Method 1
a fluorescence microscope capable of generating fluorescence by irradiating an object to be observed that is dyed with a fluorescent dye reagent, with an excitation ray
Implementation Method 2
the fluorescence intensity of the fluorescent substance drops (that is, fluorescence fading occurs or its apparent quantum yield drops) depending on the intensity of the excitation ray and the passage of the irradiation time
Data Source
AI summary
Provided is an information processing apparatus (100) including: an image acquiring unit (112) that acquires captured image information of a sample (20) dyed with a fluorescent dye reagent (10), an information acquiring unit (111) that acquires information related to the fluorescent dye reagent (10), a correcting unit (131) that corrects the luminance of the captured image information using a fluorescence fading coefficient that represents the rapidness at which the fluorescence intensity of the fluorescent dye reagent (10) drops, the fluorescence fading coefficient being included in the fluorescent dye reagent (10), and a calculating unit (132) that calculates information corresponding to fluorescent molecules in the captured image information, using the corrected luminance.