Fluorescence Receiving Apparatus with Feedback Hologram Correction
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Solution Overview
Problem
Fluorescence scanning microscopes using spatial light modulators face challenges in controlling the intensity of multiple light spots at focusing positions due to variations in the modulator and detector sensitivity, leading to non-uniform fluorescence detection.
Innovation Solution
A fluorescence receiving apparatus and method that employs a spatial light modulator to generate and correct holograms based on sensitivity information and fluorescence intensities, ensuring uniform intensity levels across multiple spots by feedback correction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If a spatial light modulator is used to generate multiple light spots on a specimen, then the number of simultaneously scanned points increases and image acquisition time is reduced, but the intensities of the multiple spots become non-uniform due to variation in the modulator and detector sensitivity
Solution Approach 1:
The patent implements feedback control by measuring the actual intensities of multiple light spots at focusing positions and using this information to adjust the hologram displayed on the spatial light modulator. The control unit modifies the phase distribution in the hologram based on detected intensity variations, creating a closed-loop system that compensates for detector sensitivity variations and modulator non-uniformity, thereby achieving uniform spot intensities while maintaining high-speed multi-point scanning
Solution Approach 2:
The patent changes the phase distribution parameter of the hologram displayed on the spatial light modulator based on detected intensity variations. By dynamically adjusting the phase values at different pixel locations in the hologram, the system compensates for intensity non-uniformities in the multiple light spots, enabling uniform illumination across all focusing positions while maintaining high scanning speed
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively controls the intensity of multiple light spots and fluorescence emission, reducing the impact of detector sensitivity variations and achieving uniformity in fluorescence detection.
Implementation Method 1
a spatial light modulator to which the excitation light is input, which expresses a first hologram, thereby to modulate at least either one of a phase and an amplitude of the excitation light
Implementation Method 2
multiple spots can be generated on focusing points of an objective lens when a CGH (Computer Generated Hologram) is presented on the SLM
Implementation Method 3
a focusing optical system provided as a subsequent stage to the spatial light modulator and configured to focus the modulated light to a specimen
Implementation Method 4
fluorescence excited from a specimen also forms a plurality of spots
Implementation Method 5
a fluorescence detector for detecting fluorescence generated by focus of the modulated light to the specimen
Data Source
AI summary
A fluorescence receiving apparatus comprises an excitation light source, a spatial light modulator of a phase modulation type for phase-modulating excitation light to obtain phase-modulated light, a focusing optical system configured to focus the phase-modulated light to a specimen, a specimen stage for supporting the specimen, a fluorescence receiver for receiving fluorescence generated by focus of the phase-modulated light to the specimen, a control unit for displaying a first CGH on the spatial light modulator, and a correction unit for correcting the first CGH. The correction unit comprises a receiver-specific sensitivity information storage preliminarily acquiring and storing sensitivity information per reception position specific to the fluorescence receiver, and a second hologram generator for correcting the first CGH, based on intensities of the fluorescence and the sensitivity information, to generate a second CGH. The control unit displays the second CGH on the spatial light modulator.