Fluorescence Intensity Analysis System for Liveware Flow Mapping
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Solution Overview
Problem
Current fluorescence testing methods are invasive and limited to local areas, failing to accurately perceive the flow range of fluorescence, especially in liveware research.
Innovation Solution
A system comprising first and second fluorescence intensity detection devices, connected to a picture processing device, which successively detects and synthesizes fluorescence intensity ranges at different timing points to create a synthesized picture, allowing for image processing to calculate and mark the fluorescence target range.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If fluorescence material is targeted to a general target area using a fluorescence camera for observation, then fluorescence distribution and intensity can be observed, but the observation is limited to a local area and the flow range of fluorescence cannot be accurately perceived
Solution Approach 1:
The patent divides the fluorescence detection process into multiple segments by using multiple fluorescence detection devices positioned at different locations. Each device detects fluorescence intensity in its specific detection area, and the picture processing device integrates these segmented detections to form a comprehensive view of the entire fluorescence flow range, thereby expanding the effective observation area while maintaining accurate flow range perception.
Solution Approach 2:
The patent transitions from single-point or single-area detection to multi-dimensional spatial detection by arranging multiple fluorescence detection devices in different spatial positions. This dimensional expansion allows simultaneous detection of fluorescence intensity across multiple areas, enabling accurate perception of the overall fluorescence flow range while maintaining detailed local observation capabilities.
2Measurement precision
If invasive methods or tissue slicing are used for fluorescence observation in liveware, then fluorescence distribution can be observed, but the method becomes invasive and complex
Solution Approach 1:
The patent replaces invasive mechanical methods (tissue slicing and physical intervention) with non-invasive optical detection. Multiple fluorescence detection devices optically detect fluorescence intensity from the liveware without requiring physical penetration or tissue removal, thereby maintaining accurate fluorescence distribution observation while eliminating the complexity and harm of invasive procedures.
Solution Approach 2:
The patent introduces fluorescence detection devices as intermediary tools that indirectly observe fluorescence distribution through optical signals rather than direct physical intervention. This intermediary approach allows accurate measurement of fluorescence distribution in liveware without requiring invasive access or tissue slicing, simplifying the overall system while maintaining measurement precision.
3Loss of information
If multiple fluorescence detection devices are used to detect fluorescence intensity at different timing points, then comprehensive fluorescence distribution can be analyzed, but the system complexity increases
Solution Approach 1:
The patent merges the data from multiple fluorescence detection devices through a centralized picture processing device. This device receives fluorescence intensity data from all detection devices, correlates the data according to timing information, and synthesizes a comprehensive fluorescence distribution analysis. This merging approach maintains complete information while managing system complexity through centralized integration rather than distributed processing.
Solution Approach 2:
The patent designs the picture processing device with multi-functional capabilities: it receives data from multiple detection devices, performs timing correlation analysis, synthesizes fluorescence distribution images, and provides comprehensive analysis. This universal device handles all processing tasks that would otherwise require multiple specialized systems, thereby reducing overall system complexity while maintaining complete fluorescence distribution information.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables convenient fluorescence distribution analysis by overcoming the limitations of invasive and localized fluorescence testing, providing a comprehensive view of fluorescence distribution.
Implementation Method 1
a fluorescence material is targeted to a general target area, and then a fluorescence camera is employed to observe the distribution and intensity of the fluorescence
Data Source
AI summary
A fluorescence intensity analyzing and fluorescence image synthesizing system and method are disclosed. The first fluorescence intensity detection device successively detects the plurality of first fluorescence intensities according to the first timing and the second fluorescence intensity detection device successively detects the plurality of second fluorescence intensities according to the second timing, and then the picture processing device analyzes the first and second timings and synthesizes the first and second fluorescence intensity ranges into the synthesized picture according to the fluorescence intensities, whereby the image processing technology may be used to calculate the fluorescence target range and thus mark the fluorescence target range.