Fluorescence Microscope Identification Filter for Biomarker Analysis
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Solution Overview
Problem
Conventional fluorescence microscopes require multiple light sources and cameras to identify different types of samples, limiting the number of samples that can be analyzed simultaneously due to overlapping fluorescence spectra, increased device size, and reduced sensitivity.
Innovation Solution
A fluorescence microscope equipped with an identification filter that blocks specific fluorescence spectra, allowing for the simultaneous analysis of multiple samples by dispersing the remaining spectra into an elliptical shape for identification, reducing the need for multiple light sources and improving detection area and sensitivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple light sources and cameras are used to identify different types of samples, then the number of samples that can be analyzed simultaneously increases, but the device size increases and complexity increases
Solution Approach 1:
The patent segments the fluorescence spectrum into distinct wavelength ranges using wavelength-specific filters. By dividing the spectral range into segments (e.g., blue, green, red fluorescence ranges), the system can identify multiple sample types using a single light source and detector, eliminating the need for multiple light sources and cameras while maintaining the ability to analyze multiple samples simultaneously.
Solution Approach 2:
The patent implements a universal detection system where a single light source and single camera can detect multiple types of fluorescence emissions by using wavelength-specific filters. The filter acts as a multi-functional component that enables the single detector to perform multiple detection functions for different sample types, reducing device complexity while maintaining versatility.
2Adaptability or versatility
If multiple light sources and cameras are used to identify different types of samples, then the number of samples that can be analyzed simultaneously increases, but the cost increases
Solution Approach 1:
The patent segments the fluorescence spectrum into distinct wavelength ranges using wavelength-specific filters. By dividing the spectral range into segments (e.g., blue, green, red fluorescence ranges), the system can identify multiple sample types using a single light source and detector, eliminating the need for multiple light sources and cameras while maintaining the ability to analyze multiple samples simultaneously.
Solution Approach 2:
The patent implements a universal detection system where a single light source and single camera can detect multiple types of fluorescence emissions by using wavelength-specific filters. The filter acts as a multi-functional component that enables the single detector to perform multiple detection functions for different sample types, reducing device complexity while maintaining versatility.
3Adaptability or versatility
If fluorescence spectra from multiple fluorophores are used simultaneously, then the types of samples to be identified increases, but the detection time increases
Solution Approach 1:
The patent segments the detection process by using wavelength-specific filters to separate different fluorescence emissions in the spectral domain. This allows simultaneous detection of multiple fluorophores with overlapping spectra through parallel spectral filtering, enabling identification of multiple sample types without increasing detection time.
Solution Approach 2:
The patent introduces the spectral dimension as a new way to distinguish between different fluorophores. Instead of relying on spatial separation or sequential detection, the system uses wavelength-specific filters to create spectral signatures that allow simultaneous identification of multiple samples, adding a dimensional approach to the detection problem.
4Device complexity
If a single fluorescence image is obtained according to light irradiated to the sample, then the device structure is simple, but various types of fluorescent dyes require various wavelengths of light sources and cameras
Solution Approach 1:
The patent segments the detection system by introducing wavelength-specific filters that divide the spectral range into distinct segments. This allows a single simple device structure to handle multiple fluorescent dye types by spectrally separating their emissions, eliminating the need for multiple light sources and cameras while maintaining the ability to identify various sample types.
Solution Approach 2:
The wavelength-specific filter acts as an intermediary component between the single light source and the single detector. This intermediary element enables the simple device structure to achieve complex identification capabilities by mediating the interaction between light and fluorophores, allowing spectral separation without adding multiple light sources or detectors.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate identification of multiple biomarkers with improved detection area and sensitivity, allowing for the use of various fluorophores with overlapping spectra and reducing the number of excitation light sources required.
Implementation Method 1
A fluorescence microscope is an optical microscope for imaging using fluorescence, and may detect and image long-wavelength light in which light absorbed by a fluorescent material is emitted in the form of fluorescence
Implementation Method 2
The fluorescent material in the sample absorbs the specific-wavelength light and emits long-wavelength light in the form of fluorescence
Implementation Method 3
a spectroscopic means for dispersing the fluorescence spectrum
Data Source
AI summary
Provided are a fluorescence microscope for identifying a biomarker capable of easily identifying a type of biomarker through fluorescence analysis and a biomarker identification method using the same. The present disclosure relates to a fluorescence microscope capable of identifying a biomarker in a fluorescence microscope for analyzing fluorescence emitted from a sample, wherein a detection unit of the fluorescence microscope is provided with an identification filter that blocks some of fluorescence spectra incident to the detection unit.