Fluorescence Intensity Normalization for Changing Optics Settings
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Solution Overview
Problem
Existing fluorescence microscopy and endoscopy devices face challenges in accurately determining fluorophore concentration due to the influence of optics setting parameters, which affect fluorescence intensity.
Innovation Solution
A method and device that automatically adjust exposure control parameters, such as detector gain and light intensity, based on optics setting parameters like working distance and magnification, using a controller with calibration data or interpolation, to maintain constant fluorescence image brightness.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If optics setting parameters are changed to adapt to different observation conditions, then the observation capability and versatility are improved, but the fluorescence intensity measurement accuracy deteriorates due to the functional relationship between sensitivity and optics settings
Solution Approach 1:
The patent applies parameter changes by systematically varying optics setting parameters (such as objective lens numerical aperture, excitation power, detector gain) and recording the corresponding fluorescence intensity measurements. These parameters are then used in a normalization calculation to compensate for their effect on intensity measurements, allowing accurate quantification across different optical configurations.
Solution Approach 2:
The patent implements feedback by measuring the actual fluorescence intensity under each optics setting and using this information to calculate normalization factors. The system continuously monitors and adjusts for optics parameter effects, creating a closed-loop approach that maintains measurement accuracy despite changes in observational conditions.
2Ease of operation
If automatic adjustment of exposure control parameters is implemented to maintain constant brightness, then the ease of operation is improved, but the device complexity increases due to additional control mechanisms
Solution Approach 1:
The patent applies self-service by enabling the system to automatically determine and adjust exposure control parameters based on the current optics settings. The controller autonomously calculates the required adjustments to detector gain, exposure time, or light source intensity to maintain consistent fluorescence image brightness without user intervention, making the system self-regulating.
Solution Approach 2:
The patent implements preliminary action by pre-calculating or pre-storing the relationships between optics setting parameters and required exposure control adjustments. When an optics parameter is changed, the system has already prepared the corresponding exposure parameter adjustments, enabling immediate automatic compensation without complex real-time calculations during observation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method and device effectively counterbalance the effects of changing optics settings, ensuring consistent fluorescence intensity and fluorophore concentration measurement without user interaction.
Implementation Method 1
a light source configured to emit illumination light for exciting fluorophores to emit a fluorescence light
Data Source
Figure 1
AI summary
The invention relates to a method, in particular a fluorescence microscopy or endoscope imaging method for intensity normalization, a non-transient computer readable storage medium (101) and a controller (51) for an endoscope (3) or microscope device (5). In the prior art, common observation devices (1) as surgical microscopes, endoscopes (3) or microscopes (5) have the disadvantage that it is ambiguous whether a detected intensity (97) is due to fluorophore (29) concentration or due to optics setting parameters (73). The inventive method overcomes said disadvantages by automatically adjusting at least one exposure control parameter (83) if at least one optics setting parameter (73) is changed. The inventive non-transient computer readable storage medium (101) is adapted to run a program for executing the inventive method and the inventive controller (51) comprises an input section (67) for receiving optics setting data (71); an output section (69) for outputting exposure control data (81); and a determination module (53) for determining at least one exposure control data (81) dependent on at least one optics setting data (71).