Two-Dimensional Fluorescence Plot Correction for Multi-Dye Spectral Leakage
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Solution Overview
Problem
Existing fluorescence analysis methods struggle to accurately correct for fluorescence intensity leakage when multiple fluorescent dyes are used, leading to inaccurate two-dimensional plots due to mismatched spectral references or dye deterioration, especially with large numbers of dye types.
Innovation Solution
An information processing apparatus and method that generates a two-dimensional plot group based on correction processing, allowing users to intuitively modify spectral references through user operations on displayed plots, facilitating easy grasp of correction influences.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If spectral reference modification is performed to correct fluorescence leakage, then measurement precision is improved, but device complexity increases due to difficulty in grasping influence on unmixing processing
Solution Approach 1:
The patent creates a copy of the spectral reference waveform and displays it visually on the UI. This visual copy allows users to see the spectral reference shape and understand how modifications will affect unmixing results, without directly manipulating the complex spectral reference data structure. The visual representation serves as an intermediary that simplifies the interaction between user and spectral reference.
Solution Approach 2:
The patent employs temporary visual displays of spectral references that can be easily created, modified, and discarded. These visual representations are lightweight and do not require permanent storage or complex management, allowing users to experiment with different spectral reference shapes without significant overhead.
2Adaptability or versatility
If the number of fluorescent dyes is increased to enhance analysis capability, then adaptability is improved, but ease of operation deteriorates due to difficulty in grasping correction influence
Solution Approach 1:
The patent segments the overall unmixing process into individual fluorescent dye components. Each fluorescent dye's spectral reference is displayed and adjustable independently, allowing users to focus on one dye at a time rather than being overwhelmed by the entire multi-dye system. This segmentation makes the operation easier while maintaining support for many dye types.
Solution Approach 2:
The patent adds a visual dimension to spectral reference manipulation. Instead of working solely with numerical spectral data in multidimensional space, the system projects spectral references into a visual format that users can see and intuitively understand, adding a graphical dimension that simplifies interaction with complex multi-dye systems.
3Manufacturing precision
If spectral reference waveform modification is performed to match actual fluorescent dye characteristics, then manufacturing precision is improved, but ease of operation worsens due to complexity of waveform modification
Solution Approach 1:
The patent creates visual copies of spectral reference waveforms that can be displayed and compared. Users can see the actual waveform shape and make adjustments based on visual feedback rather than manipulating abstract numerical data, significantly easing the modification process while maintaining precision.
Solution Approach 2:
The patent implements visual feedback mechanisms that show users the current spectral reference waveform and the effects of proposed modifications. This feedback loop allows users to iteratively adjust waveforms while immediately seeing the impact on accuracy, making the precision adjustment process easier and more intuitive.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables intuitive and effective correction of fluorescence data analysis results, even with numerous fluorescent dyes, ensuring accurate two-dimensional plots and facilitating rapid analysis adjustments.
Implementation Method 1
a particle population such as cells is labeled with a fluorescent dye, each particle of the particle population is irradiated with a laser beam, and the intensity and/or pattern of fluorescence generated from the excited fluorescent dye is measured
Data Source
AI summary
To provide a technique for easily grasping an influence of correction processing on a two-dimensional plot.The present technology provides an information processing apparatus including a two-dimensional plot generation unit that generates a two-dimensional plot on the basis of fluorescence data obtained from a particle population labeled with a plurality of fluorescent bodies, and generates a two-dimensional plot group regarding any one fluorescent body of the plurality of fluorescent bodies and each of all the other fluorescent bodies on the basis of correction processing on the two-dimensional plot. Furthermore, the present technology also provides an information processing method including a two-dimensional plot group generation process of generating the two-dimensional plot group, and a program for causing an information processing apparatus to execute a two-dimensional plot group generation process of generating the two-dimensional plot group.