Fluorescence Detection Using Dual-Surface Reflector for Spot Alignment
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Solution Overview
Problem
Fluorescence light detection devices with separate fibers for excitation and fluorescence light suffer from misalignment between the excitation light spot and the fluorescence light spot, leading to inefficient capture of high-intensity fluorescence signals.
Innovation Solution
Incorporating a reflective member with two reflective surfaces between the excitation light fiber and the fluorescence light fiber, positioned between the objective lens and the test object, to ensure coincidence of the excitation and fluorescence light spots, thereby improving the detection efficiency of fluorescence light.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If separate fibers are used for excitation light and fluorescence light, then device structure is simplified and ease of manufacture is improved, but misalignment between excitation light spot and fluorescence light spot occurs leading to reduced measurement precision
Solution Approach 1:
A reflective member is introduced as an intermediary component between the excitation light fiber and fluorescence light fiber. This reflective member redirects excitation light to coincide with the fluorescence light spot position, enabling precise alignment without requiring complex fiber positioning mechanisms, thus maintaining ease of manufacture while achieving high measurement precision.
Solution Approach 2:
The invention introduces a spatial dimension solution by using a reflective member positioned at a specific angle and location between the two fibers. This allows the excitation light path to be redirected in three-dimensional space to match the fluorescence light spot, resolving the alignment issue without complicating the basic fiber-based structure.
2Device complexity
If separate fibers are used for excitation light and fluorescence light, then device complexity is reduced, but detection sensitivity deteriorates due to inability to capture high-intensity fluorescence signals
Solution Approach 1:
The reflective member serves as a simple intermediary that redirects excitation light to the correct spot without adding significant device complexity. It enables the excitation light to reach the fluorescence light spot position, ensuring that high-intensity fluorescence signals are generated and captured effectively, thereby maintaining detection sensitivity.
Solution Approach 2:
The invention maintains the segmented structure of separate excitation and fluorescence light fibers, which keeps device complexity low. The reflective member is added as a minimal component that enables precise spot alignment, allowing the system to capture high-intensity fluorescence signals without requiring a completely integrated or complex optical design.
3Measurement precision
If defocused state is induced to capture fluorescence light, then fluorescence light can be captured in overlapping region, but fluorescence light intensity is reduced
Solution Approach 1:
The reflective member acts as a precise alignment tool that directs excitation light to exactly match the fluorescence light spot position in focus. This eliminates the need for defocusing, allowing the system to capture fluorescence light at maximum intensity while maintaining proper focus, thus avoiding the intensity reduction that would result from defocusing.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enhances the intensity of detected fluorescence light and reduces optical noise, improving the precision of fluorescence light measurement by isolating the areas for excitation and fluorescence light passage, allowing for more effective capture of fluorescence signals.
Implementation Method 1
a reflective member arranged between where the excitation light emitting end and the fluorescence light incident end are located, and the objective lens, and having two reflective surfaces facing in opposite directions
Implementation Method 2
how excitation light emitted from an excitation light fiber 101 is focused by an objective lens 103 onto a sample S
Implementation Method 3
how fluorescence light emitted from a fluorescence light fiber 102 is focused by the objective lens 103 onto the sample S
Implementation Method 4
detect fluorescence light produced by the test object due to the excitation light
Data Source
AI summary
A fluorescence light detection device includes an excitation light fiber having an excitation light emitting end configured to emit excitation light; a fluorescence light fiber having a fluorescence light incident end on which fluorescence light is incident; an objective lens arranged between where the excitation light emitting end and the fluorescence light incident end are located, and a sample; and a reflective member arranged between where the excitation light emitting end and the fluorescence light incident end are located, and the objective lens, and having two reflective surfaces facing in opposite directions. The two reflective surfaces of the reflective member are positioned between an optical axis of the excitation light fiber and an optical axis of the fluorescence light fiber.