Relative Fluorescence Measurement Using Stable Resin Standard Plates
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Solution Overview
Problem
Existing methods for measuring the relative fluorescence intensity of near-infrared fluorescent dyes in resin compositions are unreliable due to variations in dye dispersion and fluorescence intensity, which are affected by measurement devices and conditions, leading to inconsistent results.
Innovation Solution
A method using plate-shaped or film-shaped molded bodies of resin compositions containing near-infrared fluorescent dyes, along with blank plates without dyes, to measure fluorescence intensity relative to a standard plate, ensuring uniform dispersion and stable emission, utilizing specific compounds like those represented by General Formulas (I1) to (I4) for improved accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If Rhodamine B solution is used as a standard sample for near-infrared fluorescence measurement, then the measurement can be performed, but the fluorescence intensity varies significantly over time and between measurements
Solution Approach 1:
The patent changes the chemical composition parameters of the standard sample by using a commercially available near-infrared fluorescent resin composition (containing dyes like BODIPY or DPP-based boron complexes) instead of Rhodamine B solution. This resin composition maintains stable fluorescence intensity over time and across measurements, resolving the stability issue while enabling reliable near-infrared fluorescence measurement.
2Manufacturing precision
If melt-kneading is used to disperse near-infrared fluorescent dye in resin, then uniform dispersion can be achieved, but fluorescence emission is lost due to dye decomposition at high temperatures
Solution Approach 1:
The patent changes the processing temperature parameter by using low-temperature melt-kneading methods or alternative processing techniques that do not require high temperatures. This allows the dye to be uniformly dispersed in the resin without causing thermal decomposition, thereby maintaining both dispersion uniformity and fluorescence emission stability.
Solution Approach 2:
The patent utilizes commercially available pre-formulated resin compositions that contain near-infrared fluorescent dyes already dispersed and stabilized. These compositions are designed to maintain fluorescence stability during processing and use, eliminating the need for complex dispersion processes that would compromise dye integrity.
3Measurement precision
If Rhodamine B solution is prepared fresh for each measurement, then measurement accuracy may be maintained, but the preparation process is time-consuming and variable
Solution Approach 1:
The patent adopts commercially available standard resin compositions as ready-to-use standard samples. These pre-prepared standards eliminate the need for laboratory preparation of Rhodamine B solutions, significantly reducing preparation time and variability while maintaining measurement accuracy through consistent, factory-controlled formulations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method provides a reliable and stable means to measure near-infrared fluorescence intensity with reduced variability, enabling consistent and accurate comparisons between samples.
Implementation Method 1
a method for measuring a relative fluorescence intensity of a test sample that emits near-infrared fluorescence
Data Source
AI summary
A method for measuring a relative fluorescence intensity of a test sample includes measuring fluorescence intensities F1 of a test sample at wavelength λ1, F1B of a test sample blank at wavelength λ1B, FS of a standard plate at wavelength λS, and FSB of a blank plate at wavelength λSB, respectively, and defining (F1-F1B)/(FS-FSB). The standard plate is a plate-shaped or film-shaped molded body of a resin composition obtained by melt-kneading a raw material mixture containing a near-infrared fluorescent dye and an amorphous resin. The blank plate is a plate-shaped or film-shaped molded body of a resin composition identical to the standard plate except for the near-infrared fluorescent dye. The test sample is a molded body of a resin composition containing a near-infrared fluorescent dye. The test sample blank is a molded body of a composition identical to the test sample except for the near-infrared fluorescent dye.