Fluorescence Detection Sensitivity Analysis Using Single Bead Type
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Solution Overview
Problem
In optical analysis of microparticles, existing methods require different types of beads for evaluating device performance and adjusting devices, leading to low fluorescence intensity, especially at 700 nm or less, and difficulties in separating adjacent fluorescence peaks, making accurate device evaluation and adjustment challenging.
Innovation Solution
An information processing device and system that uses a single type of beads labeled with a fluorescent dye of single intensity, acquiring fluorescence intensities at multiple light irradiation powers to calculate sensitivity information, including linearity and detection sensitivity, allowing for accurate device performance evaluation and adjustment without the need for advanced separation techniques.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple types of beads labeled with different fluorescent dyes are used to evaluate device performance, then fluorescence intensity coverage is improved, but measurement precision deteriorates due to low fluorescence intensity at 700 nm or less
Solution Approach 1:
The invention changes the parameter of light irradiation power to acquire fluorescence intensities at multiple power levels. By varying the excitation power and analyzing the relationship between power and fluorescence intensity, the system can evaluate device performance across different intensity ranges using a single bead type, thereby maintaining measurement precision while achieving versatile fluorescence coverage.
2Reliability
If multiple types of beads are used for device evaluation and adjustment, then evaluation comprehensiveness is improved, but device complexity increases due to needing to secure two types of beads
Solution Approach 1:
The invention makes a single type of bead serve multiple functions by acquiring fluorescence intensities at multiple light irradiation powers. This allows one bead type to provide comprehensive evaluation data for both device performance assessment and adjustment, eliminating the need to manage separate bead types for different purposes and thereby reducing device complexity.
Solution Approach 2:
The invention merges the functions of multiple bead types into a single bead type by combining measurements at different light irradiation powers. Instead of using separate beads for different evaluation purposes, the system combines multiple power-level measurements from one bead type to achieve comprehensive device evaluation and adjustment capabilities.
3Ease of operation
If beads with single fluorescence intensity are used for device adjustment, then ease of operation is improved, but measurement precision deteriorates due to inability to evaluate linearity and sensitivity
Solution Approach 1:
The invention applies periodic action by acquiring fluorescence intensities at multiple discrete light irradiation power levels. This systematic variation of excitation power creates a series of measurements that can be analyzed to determine linearity and sensitivity, thereby enabling precise device evaluation while maintaining the operational simplicity of using a single bead type.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables highly accurate device performance evaluation and adjustment using the same type of beads, improving fluorescence detection sensitivity and eliminating the need for complex peak separation, while accurately reflecting device state through background signal analysis.
Implementation Method 1
a fluorescence signal from a sample including particles labeled with a fluorescent dye
Data Source
AI summary
It is aimed to provide a technology that enables highly accurate device performance evaluation and device adjustment in optical analysis of microparticles, using the same type of beads. The present technology provides an information processing device including an information processing unit that acquires a plurality of fluorescence intensities at a plurality of light irradiation powers for a fluorescence signal from a sample including particles labeled with a fluorescent dye having a single fluorescence intensity, recognizes an intensity range of each of the plurality of fluorescence intensities detected on the basis of a fluorescence intensity balance of the sample, and calculates information relating to sensitivity of a fluorescence detection unit.