Fluorescence Signal Detection Isolation from Well Interference
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Solution Overview
Problem
Existing fluorescence signal detection methods face interference from material emissions in sample analysis wells, making it difficult to distinguish and measure fluorescence signals emitted by reaction media accurately, which affects the precision of analyte concentration determination.
Innovation Solution
A method and system that involve illuminating the analysis assembly from multiple positions before and after introducing the reaction medium, detecting fluorescence signals from both surfaces of the well, and performing calculations to isolate the signal emitted solely by the reaction medium, using a movable light source and signal processing to account for interference from well materials.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If fluorescence signals are detected from the reaction medium in the well, then analyte concentration can be determined, but interference from well material fluorescence emissions reduces measurement precision
Solution Approach 1:
The patent applies preliminary action by performing a background measurement of the well material fluorescence before introducing the reaction medium. This pre-measurement allows the system to capture and store the interference signal characteristics, which are then subtracted from the subsequent measurement containing both well material and reaction medium fluorescence, thereby isolating the reaction medium signal and improving measurement precision.
Solution Approach 2:
The patent extracts the harmful well material fluorescence signal from the total measured signal by performing a background subtraction operation. The system separately measures the well material fluorescence (without reaction medium) and then mathematically removes this component from the combined measurement, leaving only the reaction medium fluorescence signal for analyte concentration determination.
2Reliability
If fluorescence signals are measured to determine analyte presence, then sample analysis is achieved, but interfering fluorescence from foreign substances and well materials complicates signal interpretation
Solution Approach 1:
The system performs preliminary background characterization by measuring fluorescence from the well material alone before adding the reaction medium. This preliminary measurement establishes a baseline that accounts for well material interference, enabling more reliable detection of analyte-specific fluorescence signals in subsequent measurements.
Solution Approach 2:
The patent extracts the interference component from the total fluorescence signal by subtracting the pre-measured well material fluorescence from the combined measurement. This extraction process isolates the reaction medium fluorescence, removing the harmful interference from well materials and foreign substances, thereby improving analyte detection reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the precision of fluorescence signal detection by isolating the reaction medium's signal from well material interference, allowing for accurate determination of analyte concentration in samples.
Implementation Method 1
a light source, movable along a surface S1 of the well, also called first surface S1 of the well, to illuminate the analysis assembly by means of excitation beams
Implementation Method 2
a detection device to detect the fluorescence emissions coming from the analysis assembly
Data Source
AI summary
A method and system incorporating a process for analyzing a sample to be tested to determine the presence of or to quantify an analyte in the sample. The process employs a reaction which produces a reaction medium derived from the sample that possesses fluorescence properties, in response to illumination by a light source. The process includes illuminating the sample and detecting a fluorescent signal, introducing the reaction medium, and illuminating the sample and detecting a second fluorescent signal from the sample. The process further includes processing the two signals to determine which portion of the fluorescent signal is produced solely by the reaction medium.