Fluorescence Tomography Using Line Illumination for Faster Reconstruction
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Solution Overview
Problem
Traditional fluorescence imaging tomography using point sources is time and processor intensive, necessitating improved methods for reduced data acquisition and image reconstruction time while maintaining accuracy and resolution.
Innovation Solution
The use of line sources and line illumination patterns, combined with advanced image reconstruction techniques, to project light patterns onto a subject and acquire intensity values from opposite sides, enabling efficient image generation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If point sources are used for fluorescence imaging tomography, then image reconstruction accuracy is improved, but data acquisition time and processing time increase
Solution Approach 1:
The patent segments the illumination pattern into multiple line sources arranged in a grid pattern, where each line source illuminates a specific region of the subject. This segmentation allows parallel data acquisition from multiple regions simultaneously, reducing total acquisition time while maintaining reconstruction accuracy through the combined information from all line sources.
Solution Approach 2:
The patent merges multiple line source measurements into a comprehensive image reconstruction process. By combining the fluorescence signals from multiple line sources that illuminate different regions, the system achieves complete coverage of the subject volume, maintaining quantitative accuracy while reducing the need for sequential point-by-point scanning.
2Measurement precision
If point sources are used for fluorescence imaging tomography, then image reconstruction accuracy is improved, but processor time increases
Solution Approach 1:
The reconstruction process is segmented to handle data from multiple line sources independently and then combined. This allows for optimized processing of each line source dataset and parallel computation strategies, reducing overall processor time while maintaining the quantitative accuracy needed for precise fluorophore localization.
Solution Approach 2:
The patent changes the illumination geometry parameter from point sources to line sources, which fundamentally alters the data acquisition pattern and reconstruction algorithm requirements. This parameter change enables more efficient data collection that requires less computational processing to achieve the same reconstruction quality.
3Loss of time
If line sources are used for fluorescence imaging tomography, then data acquisition time is reduced, but illumination pattern complexity increases
Solution Approach 1:
The complex illumination pattern is segmented into discrete line sources arranged in a systematic grid pattern. Each line source can be independently controlled and activated, simplifying the overall system architecture despite the multiple illumination positions. This segmentation makes the complex pattern manageable through modular control of individual line sources.
Solution Approach 2:
The line source array system is designed to be universal, where the same physical array can generate multiple different illumination patterns by selectively activating different line sources. This multi-functionality allows the system to adapt to different imaging requirements without changing the fundamental hardware configuration, reducing overall system complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces data acquisition and reconstruction time while maintaining quantitative accuracy and resolution, improving signal-to-noise ratio and reconstruction quality.
Implementation Method 1
detecting light emitted from fluorophores in the subject at a different wavelength from the emission wavelength
Data Source
AI summary
A method for fluorescence imaging tomography includes placing at least one subject on an imaging platform. The method further includes controlling light emanating from a light source to project an illumination pattern comprising at least one line onto the at least one subject. The method further includes acquiring, at a plurality of locations on a detector, light intensity values at excitation wavelengths resulting from the projecting of the illumination pattern onto the at least one subject. The method further includes acquiring, at the plurality of locations on the detector, light intensity values at emission wavelengths resulting from a fluorescence response of fluorescent sources within the at least one subject to the projecting of the illumination pattern the at least one subject. The method further includes generating an image of the fluorescent sources within the at least one subject based on the acquired light intensity values and outputting the image.