Fluorescent Cellulose Particles for Immunochromatography
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Solution Overview
Problem
Existing fluorescent nanoparticles used in immunochromatography kits face issues such as poor deployability, aggregation during storage, and inadequate color development, leading to clogging and false positives.
Innovation Solution
The development of fluorescent cellulose particles, which include cellulose particles, a fluorescent dye compound, and a heterocyclic compound within specific content ranges, improves deployability while maintaining color development properties and dispersion stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If fluorescent nanoparticles are used as detection micro particles, then color development properties and sensitivity are improved, but the particles aggregate during storage, resulting in clogging and false positives
Solution Approach 1:
The patent combines fluorescent dye compounds with cellulose particles to create composite fluorescent cellulose particles. This composite structure leverages the fluorescent properties of the dye while utilizing cellulose's stability and resistance to aggregation, thereby maintaining both high detection sensitivity and particle dispersion stability during storage
Solution Approach 2:
The patent optimizes specific parameters including the fluorescent dye content (1-40% by mass), cellulose particle size (50-500 nm), and surface treatment conditions to achieve the desired balance between fluorescence intensity and anti-aggregation properties, preventing clogging while maintaining sensitivity
2Illumination intensity
If the introduction amount of fluorescent dye compound is increased, then color development properties are improved, but the particles aggregate during storage
Solution Approach 1:
The patent establishes an optimal range for fluorescent dye content (1-40% by mass) and cellulose particle size (50-500 nm) to achieve sufficient color development while preventing aggregation. This parameter optimization ensures that the fluorescent signal is strong enough for sensitive detection without compromising particle stability
Solution Approach 2:
By creating a composite structure where fluorescent dye is incorporated into cellulose particles, the patent achieves enhanced color development properties while the cellulose matrix provides structural integrity and prevents aggregation, even at optimized dye concentrations
3Ease of operation
If colored micro particles are used to facilitate visual determination, then detection is simplified, but the particles have limited color development properties and easy discoloration
Solution Approach 1:
The patent replaces conventional colored particles with fluorescent particles that utilize optical fluorescence properties instead of relying on absorption-based color. This substitution enables visual determination through fluorescence detection while providing superior color development properties and resistance to discoloration compared to traditional colored latex or colloidal metal particles
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The improved fluorescent cellulose particles enhance deployability, reduce background coloration, and achieve a suitable signal-to-noise ratio even near the detection limit concentration, addressing the limitations of previous technologies.
Implementation Method 1
fluorescent cellulose particles, comprising cellulose particles, a fluorescent dye compound
Implementation Method 2
R1 is a functional group having affinity with a biological material
Implementation Method 3
R2 is an ether bond to the cellulose particles
Data Source
AI summary
The present invention provides fluorescent cellulose microparticles which are capable of reducing deployment failure by improving the deployability during deployment in immunochromatography, while maintaining sufficient color developability and dispersion stability if used for immunochromatography. The present invention relates to: fluorescent cellulose particles, each of which contains a cellulose particle, a fluorescent dye compound and a heterocyclic compound represented by general formula (1) (wherein R1 represents a functional group that has an affinity for a biological substance; and R2 represents an ether bond part bonded to the cellulose particle), and which are characterized in that, per 1 g of the fluorescent cellulose particles, the content of the cellulose particles is 30% by mass to 90% by mass, the content of the fluorescent dye compound is 1% by mass to 40% by mass, and the content of the heterocyclic compound is 3% by mass to 50% by mass; and a diagnostic agent and an immunochromatography kit, each of which comprises the fluorescent cellulose particles.