Fluorescent Image Acquisition Using Intensity Ratios for Single-Color Pixels
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional methods struggle to accurately discriminate single-color pixels in fluorescent images, making it difficult to efficiently observe and analyze samples with multiple substances.
Innovation Solution
A method involving irradiation with multiple wavelength excitation lights, acquisition of fluorescent images in different optical states, and calculation of intensity ratios between these images to discriminate single-color pixels, using a fluorescence filter unit with specific wavelength ranges and an inclined filter to enhance discrimination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional fluorescent image acquisition methods are used, then fluorescent images can be obtained, but single-color pixels cannot be accurately discriminated in mixed dye environments
Solution Approach 1:
The patent segments the fluorescent image analysis into two distinct categories: single-color pixels and mixed-color pixels. By calculating intensity ratios across multiple excitation wavelengths and separating pixels based on whether their ratios match reference single-dye ratios, the method achieves accurate discrimination and classification of different pixel types in the fluorescent image
Solution Approach 2:
The patent changes the parameter of measurement by acquiring fluorescent images at multiple excitation wavelengths and calculating intensity ratios. This parameter transformation allows single-color pixels to be identified by their characteristic ratio values that match reference single-dye ratios, thereby improving discrimination accuracy in mixed dye environments
2Measurement precision
If multiple excitation lights are used to improve discrimination, then single-color pixels can be identified, but the complexity of the acquisition system increases
Solution Approach 1:
The patent employs a fluorescence filter unit with multiple reflection wavelength ranges and transmission wavelength ranges that can handle multiple excitation lights and multiple fluorescences simultaneously. This multi-functional component allows the system to acquire images under different excitation conditions without requiring separate acquisition systems for each wavelength
Solution Approach 2:
The patent combines multiple fluorescent images acquired under different excitation lights into a unified analysis framework. By merging the images and calculating intensity ratios across all excitations, the system achieves accurate single-color pixel discrimination while using a single integrated acquisition device rather than multiple separate systems
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate discrimination of single-color pixels in fluorescent images, even in mixed dye environments, allowing for effective analysis and separation of fluorescent signals.
Implementation Method 1
acquiring, with respect to each of a plurality of fluorescences corresponding to each of the plurality of excitation lights, a first fluorescent image in a first optical state and a second fluorescent image in a second optical state
Implementation Method 2
through a fluorescence filter unit having a plurality of reflection wavelength ranges and a plurality of transmission wavelength ranges
Data Source
Figure 1
Figure 2
Figure 3
AI summary
A fluorescent image acquisition system 1 includes an irradiation device 2 which irradiates a sample with each of excitation lights of a plurality of wavelength distributions; an image acquisition device 3 which acquires, with respect to each of a plurality of fluorescences corresponding to each of the plurality of excitation lights, a first fluorescent image in a first optical state and a second fluorescent image in a second optical state in which fluorescence is measured at a wavelength characteristic different from that in the first optical state through a fluorescence filter unit having a plurality of reflection wavelength ranges and a plurality of transmission wavelength ranges; and an image processing device 4 which processes a plurality of the first fluorescent images and a plurality of the second fluorescent images, in which the image processing device 4 calculates, for the first fluorescent image and the second fluorescent image, an intensity ratio that is a ratio between an intensity value of a pixel of the first fluorescent image and an intensity value of a pixel of the second fluorescent image corresponding to the pixel, and calculates the intensity ratio for each of the plurality of excitation lights, and discriminates whether the pixel is a single-color pixel based on the intensity ratio for each of the plurality of excitation lights.