Fluorescent Labeling Compound for Low-Concentration Biomolecule Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional fluorescent dyes used for labeling and detecting biomolecules have limitations in detecting low concentrations of target biomolecules effectively.
Innovation Solution
A novel compound represented by Chemical Formulas 1 and 2, which can be used to create conjugates for labeling biomolecules, enhancing detectability even at low concentrations by providing improved lower limit of detection (LOD) compared to conventional fluorescent substances.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional fluorescent dyes are used for labeling and detecting biomolecules, then the labeling and detection process can be performed, but the detectability of low concentrations of target biomolecules is insufficient
Solution Approach 1:
The patent modifies the chemical structure of fluorescent dyes by introducing specific substituents (R1-R6 groups) at defined positions on the xanthene ring system. These structural parameter changes enhance the fluorescence quantum yield and molar absorptivity, thereby improving detectability and lowering the limit of detection for biomolecules at low concentrations.
Solution Approach 2:
The patent creates composite fluorescent probe systems by conjugating the novel fluorescent dye compounds with targeting moieties such as nucleic acid probes, antibodies, or other biomolecule-specific ligands. This composite approach combines the enhanced optical properties of the new dye with the specific binding capability of the targeting moiety, achieving both high detectability and specificity for low concentration targets.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel compound allows for improved detectability of biomolecules, especially at low concentrations, making it suitable as a substitute for conventional fluorescent substances in applications like real-time PCR.
Implementation Method 1
Fluorescent dyes are used in molecular biology, cell biology, and molecular genetics as detection labels for detecting biomolecules in vivo and in vitro
Data Source
Figure 1~2
Figure 3~4
Figure 5~6
AI summary
The present invention relates to a novel compound and uses thereof, and more specifically, to a compound capable of labeling biomolecules (such as nucleic acids and proteins), a composition for labeling or detecting biomolecules containing the compound, a support for labeling or detecting biomolecules containing the compound, and a method of labeling or detecting biomolecules using the compound.