Fluorescent Biomolecule Labeling Compounds for Low-Concentration Detection
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Solution Overview
Problem
Existing fluorescent dyes for labeling and detecting biomolecules, such as nucleic acids and proteins, face challenges in achieving low detection limits, especially when target biomolecules are present at low concentrations.
Innovation Solution
Development of novel compounds represented by Chemical Formulas 1 and 2, which include reactive groups and carrier molecules, allowing for improved labeling and detection of biomolecules with enhanced detectability, even at low concentrations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional fluorescent dyes are used for labeling and detecting biomolecules, then the labeling and detection function is achieved, but the detection limit is insufficient when target biomolecules are present at low concentrations
Solution Approach 1:
The patent modifies the chemical structure of fluorescent dyes by introducing specific substituents (R1-R6 groups) at defined positions on the xanthene ring system. These structural parameter changes enhance the fluorescent properties and binding affinity, enabling detection at lower concentrations while maintaining reliability.
Solution Approach 2:
The invention creates composite fluorescent probe structures by combining the core fluorescent dye molecule with specific functional groups and substituents. This composite approach allows the molecule to simultaneously exhibit fluorescent properties and high affinity for target biomolecules, improving both detection limit and reliability.
2Measurement precision
If fluorescent dyes are used to label biomolecules, then biomolecule detection is enabled, but the detectability is reduced when biomolecule concentrations are low
Solution Approach 1:
The patent optimizes molecular parameters including substituent types, positions, and configurations on the fluorescent dye structure. These changes enhance the dye's fluorescent quantum yield and target binding affinity, enabling effective detection even when the quantity of biomolecule is low.
3Measurement precision
If conventional fluorescent substances are used, then labeling function is provided, but the limit of detection is not sufficiently low
Solution Approach 1:
The invention systematically varies structural parameters of the fluorescent dye including substituent identity, position, and stereochemistry to optimize detection sensitivity. This achieves a lower limit of detection by enhancing fluorescent signal intensity and target affinity.
Solution Approach 2:
The patent introduces specific functional groups at particular positions on the molecular structure to locally enhance binding affinity or fluorescent properties. This localized optimization improves overall detection sensitivity without compromising the entire molecular structure.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel compounds exhibit a lower limit of detection (LOD) compared to conventional fluorescent substances, enabling effective labeling and detection of biomolecules, particularly nucleic acids and proteins, at low concentrations.
Implementation Method 1
Fluorescent dyes are used in molecular biology, cell biology, and molecular genetics as detection labels for detecting biomolecules in vivo and in vitro
Data Source
AI summary
The present invention relates to a novel compound and uses thereof, and more specifically, to a compound capable of labeling biomolecules (such as nucleic acids and proteins), a composition for labeling or detecting biomolecules containing the compound, a support for labeling or detecting biomolecules containing the compound, and a method of labeling or detecting biomolecules using the compound.