Fluorescent Supramolecular Biosensor for Rapid Bacterial Detection
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Solution Overview
Problem
Current methods for detecting pathogenic microorganisms are often inaccurate, time-consuming, and require large observable organisms with distinct morphology, making it difficult to quickly and precisely detect microbes in food and environmental samples.
Innovation Solution
A fluorescent supramolecular biosensor composed of self-assembled peptides with a coiled coil structure, specifically binding to E. coli, which changes fluorescence signal upon binding to target bacteria, allowing for accurate and rapid detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If PCR method is used for detection, then detection precision is improved, but detection time increases and process complexity increases
Solution Approach 1:
The patent extracts the detection function from complex multi-step processes (PCR, microscopy) and concentrates it into a single fluorescent biosensor molecule that directly binds to target bacteria and emits signal, eliminating intermediate amplification or observation steps
Solution Approach 2:
The patent replaces mechanical/optical detection systems (microscopy requiring visible morphology) with a fluorescent chemical signal system that can detect bacteria at much lower concentrations through amplified optical emission
2Device complexity
If microscopic method is used for detection, then device complexity is reduced, but detection precision deteriorates due to requirement of large observable organisms with high density
Solution Approach 1:
The patent uses fluorescent signal emission (optical property change) as the detection mechanism, allowing detection of individual bacteria or low-density populations through fluorescent signal amplification rather than requiring visible morphological changes
3Measurement precision
If conventional biosensors are used, then selectivity for specific bacteria is improved, but stability at high temperature deteriorates
Solution Approach 1:
The patent creates a composite structure combining the fluorescent probe molecule with a thermostable protein scaffold or supramolecular assembly that maintains structural integrity at high temperatures while preserving bacterial binding capability and fluorescent signal transduction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The biosensor provides high selectivity and sensitivity for specific bacteria, maintaining stability at high temperatures and enabling detection of bacterial concentrations with superior accuracy compared to existing methods.
Implementation Method 1
a fluorescent supramolecular biosensor which shows change in fluorescence signal through binding to a specific bacterium
Implementation Method 2
The supramolecular building block may form a tetramer through self-assembly when pH is 6.0-7.0 or ionic strength is 0.01-0.3 M
Data Source
AI summary
The present disclosure relates to a fluorescent supramolecular biosensor, more particularly to a fluorescent supramolecular biosensor which shows change in fluorescence signal through binding to a specific bacterium and a method for preparing the same. The fluorescent supramolecular biosensor is advantageous in that it exhibits remarkably superior affinity and selectivity for a target bacterium.In addition, because a secondary coiled coil stereostructure is highly stabilized as the supramolecular building block is self-assembled to form the fluorescent supramolecular biosensor, the fluorescent supramolecular biosensor can be stored for a long period of time without structural change even at high temperatures.