Fluorescent Tracer Quantification in Animal Feed Additives

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Solution Overview

Problem

Existing methods for detecting feed additives in animal feeds are primarily qualitative, failing to accurately quantify the amount of additives present, especially in complex or heterogeneous samples, which hinders troubleshooting of user complaints and ensures proper distribution and bioavailability of trace elements.

Innovation Solution

Incorporating water-soluble fluorescent tracers like Fluorescein, Rhodamine, and Pyranine into the manufacturing process of feed supplements, allowing for homogeneous mixing and subsequent drying, enabling quantitative detection through fluorescence analysis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If qualitative detection methods (magnetic tracers, coded tracers, iron particles with dye) are used to detect feed additives, then presence/absence detection is achieved, but quantitative detection of the amount of additive is not possible

Engineering Contradiction:
Improvequantitative detection capabilityVSAvoiddetection system complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent changes the detection parameter from binary presence/absence to continuous quantitative measurement by using fluorescent tracers that can be detected at various concentrations. The fluorescent tracer allows measurement of the amount of additive present rather than just detecting its presence, enabling quantitative analysis through fluorescence intensity measurement.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces mechanical detection methods (magnetic separation, visual inspection of coded tracers) with optical detection using fluorescence. This substitution enables quantitative measurement through fluorescence intensity, which can be measured continuously and accurately to determine the amount of additive present in the feed sample.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Measurement precision

If fluorescent analysis is used to detect fluorophors in complex samples, then sensitivity and accuracy are improved, but quenching by other compounds and light scattering make quantitative analysis difficult

Engineering Contradiction:
Improvedetection sensitivity and accuracyVSAvoidfluorescence quenching and scattering interference
Core Design Contradiction:
Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

The patent uses a fluorescent tracer as an intermediary substance that is uniformly distributed throughout the feed sample. This tracer serves as a mediator between the detection system and the feed additive, allowing quantitative measurement without direct interaction with other feed components that would cause quenching or scattering. The tracer's fluorescence intensity directly correlates with the amount of additive present.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent ensures homogeneous distribution of the fluorescent tracer throughout the feed sample. This homogeneity is achieved by incorporating the tracer during the feed manufacturing process or by thorough mixing. Uniform distribution eliminates spatial variations in fluorescence intensity, allowing accurate quantitative measurement without interference from heterogeneous sample structures that would cause scattering.

Inventive Principle:
Principle #33Homogeneity

3Reliability

If particle-based tracers (iron particles, coded tracers) are used in feed supplements, then qualitative detection is effective, but thorough mixing is required and particle size affects distribution

Engineering Contradiction:
Improvequalitative detection reliabilityVSAvoidmixing requirements and processing complexity
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent replaces particle-based mechanical mixing and detection systems with a dissolved fluorescent tracer system. Instead of requiring thorough mechanical mixing of particles, the fluorescent tracer is dissolved or uniformly distributed at the molecular level, eliminating the need for extensive mixing operations while maintaining reliable detection.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the physical state of the tracer from solid particles to dissolved molecular form. This parameter change from particulate to dissolved state eliminates particle size issues and distribution uniformity problems associated with particles, while enabling quantitative detection through fluorescence intensity measurement.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method provides accurate and reliable quantitative detection of feed additives, ensuring proper dosing and bioavailability, with the tracers being detectable at low levels and resistant to interference, thus addressing the limitations of previous qualitative detection systems.

Implementation Method 1

fluorescent tracers like Fluorescein, Rhodamine, and Pyranine into the manufacturing process of feed supplements, allowing for homogeneous mixing and subsequent drying, enabling quantitative detection through fluorescence analysis

Methodology Applied
Scientific EffectFluorescence: Fluorescence

Data Source

PatentEP2252887A1Fluorescent dye tracer method for animal feed supplement products
Publication Date: 2010.11.24 ZINPRO CORPORATION

AI summary

Compositions for detecting and measuring quantitatively amounts of admix feed additives added to animal feed mixes, using soluble tracer fluorophors, mixed with a liquid form of the additive during the manufacturing process, and then dried; and quantitative detection methods for determining the amount of the feet additive in the animal feed.