Fluorimeter Reference Cell With Integrated Scattering Calibration
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Solution Overview
Problem
Existing methods for calibrating fluorimeters are cumbersome, require multiple components, and are time-consuming, necessitating expertise and specialized holders, while traditional wavelength validation methods are limited in scope and efficiency.
Innovation Solution
A fluorimeter reference cell with integrated scattering or reflective properties, using a solution of rare-earth materials and colloidal silica, which absorbs and scatters excitation light to provide a projected absorbance spectrum for precise calibration without additional adaptors.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional wavelength calibration methods using separate reference solutions and reflectors are used, then wavelength accuracy validation is achieved, but device complexity and ease of operation deteriorate due to requiring multiple components and specialized holders
Solution Approach 1:
The patent combines the reference solution and reflector into a single integrated cuvette by incorporating a reflective coating on the inner surface of the cuvette wall. This eliminates the need for separate reflector components and specialized holders, reducing device complexity while maintaining wavelength accuracy validation capability
Solution Approach 2:
The integrated cuvette serves multiple functions: it contains the reference solution, provides the reflective surface, and fits into standard cuvette holders. This multi-functionality eliminates the need for instrument-specific adapters and simplifies operation across different fluorimeter models
2Measurement precision
If traditional calibration methods with multiple components are used, then wavelength accuracy is validated, but ease of operation worsens due to requiring expertise and specialized holders
Solution Approach 1:
By merging the reference solution and reflector into one integrated cuvette, the patent eliminates the need for users to assemble multiple components or use specialized holders. The cuvette is simply inserted into the standard holder, making the calibration process as easy as any routine measurement
Solution Approach 2:
The integrated cuvette is designed to be self-contained and self-aligning within the standard holder, eliminating the need for user expertise in positioning or assembling components. The reflective coating is pre-positioned on the cuvette wall, providing automatic alignment
3Measurement precision
If separate reference solution and reflector are used, then wavelength accuracy is calibrated, but loss of time increases due to cumbersome setup procedures
Solution Approach 1:
The patent merges the reference solution and reflector into a single pre-assembled cuvette, eliminating the time-consuming setup procedures of assembling multiple components. The calibration can be performed quickly by simply inserting the integrated cuvette into the holder
Solution Approach 2:
The reflective coating is pre-applied to the cuvette wall during manufacturing, and the reference solution is pre-filled into the cuvette. This preliminary preparation eliminates the need for users to perform time-consuming assembly or preparation steps during calibration
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables convenient, precise wavelength calibration of fluorimeters using a single integrated component, suitable for various instruments and geometries, reducing complexity and time, and ensuring comprehensive validation across the UV, visible, and NIR spectra.
Implementation Method 1
a first material, which first material emits an absorption spectrum when an excitation light is incident upon the first material
Implementation Method 2
a second material, which second material has dispersive properties such that the absorption spectrum is scattered or reflected at an angle to the angle of incidence
Implementation Method 3
the absorption spectrum is scattered or reflected at an angle to the angle of incidence
Data Source
AI summary
A fluorimeter reference cell comprising a rare earth salt solution or other optically transparent solution with a well-defined and characteristic absorbance pattern which material emits an absorption spectrum when an excitation light is incident upon it. The absorption spectrum has a defined characteristic enabling wavelength calibration of the fluorimeter. The reference cell comprises a second material, a light scattering colloid such as colloidal silica. The dispersive properties result in the absorption spectrum being scattered or reflected at an angle to the angle of incidence of the excitation light incident on the reference cell.