Fluororesin Cell Culture Container Surface Terminal Groups
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Solution Overview
Problem
Current containers for culturing and storing mammal cells, such as pluripotent stem cells and mesenchymal stem cells, fail to effectively suppress cell adhesion to the container surface, leading to reduced cell survival rates and inability to maintain high concentrations of living cells.
Innovation Solution
A container with a surface formed from a fluororesin having one or more —CF3 terminal groups or a total number of non-fluorinated group terminals and —CF2H group terminals of 70 or less per 1×10^6 carbon atoms, specifically using tetrafluoroethylene-hexafluoropropylene-based or tetrafluoroethylene-perfluoroalkylvinyl ether-based copolymers, which reduces cell adhesion and enhances cell survival.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If conventional polymer materials (polyethylene, polypropylene, polycarbonate) are used for container surfaces, then manufacturing ease and cost are improved, but cell adhesion increases leading to reduced cell survival rates
Solution Approach 1:
The invention changes the chemical composition parameters of the polymer material by incorporating fluorinated components (polytetrafluoroethylene, polyhexafluoropropylene, or their copolymers) in specific ratios (5-50 mass%). This compositional parameter change modifies the surface properties to reduce cell adhesion while maintaining manufacturing feasibility.
Solution Approach 2:
The invention uses composite materials by combining conventional polymers (polyethylene, polypropylene, or polycarbonate) with fluorinated polymers (polytetrafluoroethylene, polyhexafluoropropylene, or their copolymers) to create a new material system that exhibits both processability of conventional polymers and anti-adhesion properties of fluorinated polymers.
2Productivity
If container surfaces are designed to promote cell adhesion, then cell culture efficiency is improved, but cell loss due to adhesion increases reducing living cell concentration
Solution Approach 1:
The invention changes the surface energy parameters of the container material by using fluorinated polymers with low surface energy. This parameter change reduces the adhesive forces between the container surface and cells, minimizing cell loss while allowing cells to remain viable in culture.
Solution Approach 2:
The invention applies local quality by using fluorinated polymer components specifically at the container surface that contacts cells. The fluorinated polymers (polytetrafluoroethylene, polyhexafluoropropylene, or their copolymers) create a localized anti-adhesion layer that prevents cell attachment at the critical interface while the bulk material maintains structural integrity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The container effectively suppresses cell adhesion and maintains high cell survival rates, enabling the administration, storage, and culture of mammal cells with high concentrations of living cells, particularly beneficial for regenerative medicine applications.
Implementation Method 1
the surface of the container in contact with the mammal cells is formed of a fluororesin having one or more —CF3 terminal groups, or a fluororesin having a total number of non-fluorinated group terminals and —CF2H group terminals in the fluororesin of 70 or less per 1×106 carbon atoms
Data Source
AI summary
When mammal cells are administered by using a container whose surface in contact with the mammal cells is formed of a fluororesin material at least partially having a —CF3 terminal group or a container whose surface in contact with the mammal cells is formed of a fluororesin material having a total number of non-fluorinated group terminals and —CF2H group terminals in the fluororesin of 70 or less per 1×106 carbon atoms, or mammal cells are stored or cultured in such a container, the cell adhesion on the container inner surface and the cell survival rate reduction can effectively be suppressed. Therefore, by using these containers, a mammal cell-containing liquid having a high concentration and a high proportion of living cells can be administered, stored or prepared, which contributes to regenerative medicine using the mammal cell-containing liquid (suspension liquid).