Fluorous Affinity Extraction for Ionic Liquid Removal
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current sample preparation methods for isolating and purifying components from aqueous samples are inefficient, requiring large quantities of purification materials and lacking in selectivity and speed, particularly when dealing with ionic liquids that can interfere with analytical processes.
Innovation Solution
A method involving the combination of an aqueous sample containing an ionic liquid with an ion exchanger composition to form a fluorous salt, followed by contact with a fluorous affinity material to extract and remove the fluorous salt, utilizing a fluorous solvent to produce a purified aqueous eluate, thereby isolating the ionic liquid and allowing for further analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional sample preparation methods are used to isolate and purify components from aqueous samples, then purification can be achieved, but large quantities of purification materials are required and the process is inefficient and slow
Solution Approach 1:
The patent changes the physical-chemical parameters of the ionic liquid by converting it from a hydrophobic form to a hydrophilic fluorous salt form through ion exchange. This parameter change enables the ionic liquid to partition into the aqueous phase, dramatically reducing the quantity of purification materials needed and improving extraction efficiency
Solution Approach 2:
The patent extracts the ionic liquid from the organic phase into the aqueous phase by converting it to a hydrophilic fluorous salt. This extraction process selectively removes the ionic liquid from the sample matrix, improving both efficiency and reducing material requirements
2Measurement precision
If conventional extraction methods are used, then components can be isolated, but selectivity is insufficient and the process lacks speed
Solution Approach 1:
The patent uses parameter changes (converting ionic liquid to fluorous salt) to achieve highly selective extraction. The fluorous salt form specifically partitions into the aqueous phase, providing superior selectivity compared to conventional methods while maintaining rapid extraction speed
Solution Approach 2:
The fluorous affinity material serves multiple functions: it provides selective extraction of the fluorous salt, enables rapid separation, and facilitates concentration of the target analyte. This multi-functionality achieves both high selectivity and fast processing speed
3Reliability
If ionic liquid is present in the aqueous sample, then cell lysis and metabolism quenching are achieved, but the ionic liquid interferes with analytical processes
Solution Approach 1:
The patent extracts the ionic liquid from the aqueous sample by converting it back to its hydrophobic form through ion exchange with the analytical sample. This removal eliminates the harmful interference with analytical processes while preserving the benefits of cell lysis and metabolism quenching
Solution Approach 2:
The patent uses an intermediary ion exchange process to separate the ionic liquid from the analytical sample. The ion exchanger acts as a mediator that selectively binds the ionic liquid, allowing it to be removed without affecting the target analytes, thus eliminating interference while maintaining analytical reliability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively removes ionic liquids from aqueous samples, enhancing the analysis of target analytes by reducing contamination and improving detection sensitivity, while minimizing the use of purification materials and simplifying the process.
Implementation Method 1
combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid
Implementation Method 2
contacting the solution with a fluorous affinity material, thereby removing fluorous salt from the solution and producing an aqueous eluate
Data Source
AI summary
A method for removing an ionic liquid from an aqueous sample is provided. In some embodiments, the method includes: (a) combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid, where at least one of the ionic liquid and the ion exchanger counterion is fluorinated; (b) contacting the solution with a fluorous affinity material, thereby removing fluorous salt from the solution and producing an aqueous eluate; and (c) collecting the aqueous eluate. In certain embodiments, the method further includes: contacting a cell with an ionic liquid composition to lyse the cell and produce an aqueous sample; and contacting the aqueous sample with a reverse phase substrate, thereby adsorbing proteins and/or lipids of the cell on the substrate. Compositions, kits and systems for practicing the subject methods are also provided.