Immunochromatographic Strip for FMDV Antibody Detection
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Solution Overview
Problem
Current methods for diagnosing foot-and-mouth disease (FMD) are time-consuming, requiring multiple steps and long incubation times, which delays diagnosis and contributes to the rapid spread of the disease, especially in highly contagious scenarios, and fail to differentiate between infected and vaccinated animals.
Innovation Solution
A rapid, one-step immunochromatographic assay using E. coli-expressed recombinant FMDV structural and non-structural proteins incorporated into a solid support for the capture of antibodies in animal body fluids, allowing for the differentiation between infected and vaccinated animals within 30 minutes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional ELISA or virus neutralization tests are used for FMD diagnosis, then detection accuracy is achieved, but diagnosis time is significantly delayed due to multiple steps and long incubation times
Solution Approach 1:
The patent combines multiple detection functions into a single immunochromatographic strip that can simultaneously detect antibodies against both structural proteins (VP1, VP2, VP3) and non-structural proteins (2C, 3A, 3B, 3C, 3D) in one step, eliminating the need for separate ELISA tests and long incubation periods while maintaining detection accuracy
Solution Approach 2:
The patent pre-incorporates multiple recombinant FMDV antigens (structural and non-structural proteins) onto the immunochromatographic strip before use, so that when a sample is applied, antibody detection occurs immediately through capillary flow without requiring separate antigen preparation or long incubation steps, thus reducing diagnosis time while preserving detection precision
2Adaptability or versatility
If traditional diagnostic methods are used, then comprehensive antibody detection is achieved, but the ability to differentiate between infected and vaccinated animals is lost due to time constraints and method limitations
Solution Approach 1:
The patent divides the detection into distinct segments by incorporating separate antigen lines for structural proteins (VP1, VP2, VP3) and non-structural proteins (2C, 3A, 3B, 3C, 3D) on the immunochromatographic strip, allowing simultaneous detection and differentiation of antibody responses to different protein types, enabling distinction between infected and vaccinated animals while maintaining rapid detection speed
Solution Approach 2:
The patent uses recombinant FMDV proteins expressed in E. coli as intermediary antigens on the strip, where structural protein antigens detect vaccination-induced antibodies and non-structural protein antigens detect infection-induced antibodies, enabling differentiation capability without compromising detection speed
3Productivity
If rapid detection is implemented, then diagnosis time is reduced, but detection sensitivity and specificity may be compromised due to simplified procedures
Solution Approach 1:
The patent optimizes parameters including the concentration and configuration of recombinant antigens on the strip, the flow characteristics of the immunochromatographic medium, and the incubation time-temperature profile to achieve rapid detection within minutes while maintaining high sensitivity and specificity through controlled antigen-antibody binding conditions
4Adaptability or versatility
If multiple antigens are detected simultaneously, then comprehensive diagnosis is achieved, but device complexity increases due to multiple components required
Solution Approach 1:
The patent merges multiple detection capabilities into a single integrated immunochromatographic strip containing multiple antigen lines (structural and non-structural proteins) that can simultaneously detect different antibody types in one application, achieving comprehensive diagnosis while simplifying the overall procedure and reducing device complexity compared to multiple separate tests
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid detection of antibodies to both structural and non-structural proteins, facilitating the identification of infected animals and differentiating them from vaccinated ones, thereby aiding in the control of FMD spread and reducing financial losses.
Implementation Method 1
a nitrocellulose membrane strip upon which are placed, and allowed to dry in separate distinct capture areas, highly purified recombinant antigens derived from FMDV and/or specific monoclonal antibodies to FMDV
Implementation Method 2
The fluid test sample flows from the distal end to the proximal end
Data Source
AI summary
A rapid immunoassay method and apparatus for detecting foot and mouth disease virus are disclosed. The method and test device permit pen-side testing of animals and provide test results within a relatively short time period. In a preferred embodiment, the method and apparatus provide a means for differentiating between FMDV-infected and FMDV-vaccinated animals.