FMDV Virus-Like Particle Test Strip for Antibody Detection
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Solution Overview
Problem
Conventional methods for detecting antibodies against serotype O foot-and-mouth disease virus are susceptible to external factors and require stringent laboratory conditions, making them inaccurate, unstable, expensive, and difficult to operate.
Innovation Solution
A method for preparing serotype O foot-and-mouth disease virus-like particles and a test strip using a specific vector construction and expression process, combined with a colloidal gold-based detection system, allowing for accurate and efficient detection in normal conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods (CFT, VNT, agglutination test, immunodiffusion, ELISA) are used for detecting antibodies against serotype O FMDV, then detection can be performed, but the methods are susceptible to external factors and require stringent laboratory conditions, making them inaccurate, unstable, expensive, and difficult to operate
Solution Approach 1:
The patent uses virus-like particles (VLPs) as copies of the actual FMDV virus. These VLPs replicate the viral structure and antigenic properties without containing genetic material, allowing them to serve as detection targets that mimic the virus while being safer and easier to handle in laboratory settings
Solution Approach 2:
The patent extracts and removes the genetic material (RNA) from the FMDV virus, retaining only the structural proteins that form the virus-like particles. This extraction eliminates the hazardous viral genome while preserving the antigenic properties needed for detection, thereby reducing laboratory safety requirements and external factor susceptibility
2Productivity
If conventional detection methods are used, then antibody detection is possible, but the detection period is long and the processes are complex
Solution Approach 1:
The virus-like particles are pre-assembled in the laboratory before detection is needed. This preliminary preparation of standardized VLPs with known antigenic properties allows for rapid detection assays without the need for time-consuming viral cultivation or preparation steps during the actual detection process
Solution Approach 2:
The patent replaces complex mechanical and procedural steps of conventional detection methods with a simplified immunoassay system using VLPs. The detection process substitutes elaborate viral neutralization and complement fixation procedures with direct antigen-antibody binding reactions that can be performed rapidly under mild conditions
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method provides a stable, inexpensive, and easy-to-operate solution for detecting serotype O foot-and-mouth disease antibodies, reducing the detection period and improving accuracy compared to conventional methods.
Implementation Method 1
assembling to yield serotype O foot-and-mouth disease virus-like particles
Implementation Method 2
a test strip for detecting serotype O foot-and-mouth disease comprising a bottom board, and a detection layer disposed on the top of the bottom board... an immuno-gold pad is disposed at the other side of the detection layer close to the detection line
Data Source
AI summary
A method for preparing serotype O foot-and-mouth disease virus-like particles, the method including: construction of small ubiquitin-like modifier fusion expression vector, construction of recombinant expression vectors, construction of recombinant co-expression vector, expression and purification of proteins, and in-vitro assembly of serotype O foot-and-mouth disease virus-like particles. The disclosure also provides a test strip for detecting serotype O foot-and-mouth disease including a bottom board, and a detection layer disposed on the top of the bottom board. A detection line and a control line are disposed on the detection layer. An absorbent layer is disposed at one end of the detection layer close to the control line, and an immuno-gold pad is disposed at the other side of the detection layer close to the detection line. A sample pad is disposed on the top of the immuno-gold pad.