Forward Breeding Method for Hybrid Backcrossing

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Solution Overview

Problem

Current crop breeding methods, such as advanced cycle breeding, face limitations in generating genetically diverse selection populations with high mean performance and genetic variability, particularly when using inbred parents, which can lead to reduced genetic diversity and limited recombination.

Innovation Solution

The method involves selecting a donor parent with a trait of interest and crossing it with hybrid parents, followed by recurrent backcrossing to introduce desired genes and alleles into genetically diverse selection populations, allowing for ongoing recombination and maintaining trait phenotypic values.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If inbred parents are used in advanced cycle breeding, then breeding efficiency is improved, but genetic diversity is reduced

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidgenetic diversity
Core Design Contradiction:
ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent combines the advantages of both inbred and hybrid parents by using inbred parents for controlled crosses while incorporating hybrid parents in subsequent backcrossing operations. This merging approach maintains breeding efficiency through controlled inbred crosses while restoring genetic diversity through hybrid backcrossing, thereby resolving the contradiction between breeding efficiency and genetic diversity.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent implements a dynamic breeding scheme that transitions from static inbred parent usage to a more flexible approach incorporating multiple generations of backcrossing with hybrid parents. This dynamic adjustment allows the breeding program to adapt genetic diversity levels according to specific breeding goals, resolving the contradiction by making the system flexible rather than fixed.

Inventive Principle:
Principle #15Dynamics

2Speed

If self-fertilization is used at each generation, then inbred line development is accelerated, but recombination is limited

Engineering Contradiction:
Improveinbreeding rateVSAvoidrecombination capability
Core Design Contradiction:
SpeedVSAdaptability or versatility

Solution Approach 1:

The patent implements periodic alternation between self-fertilization generations (for rapid inbreeding) and cross-fertilization generations (for recombination). This periodic switching allows the breeding program to achieve rapid inbreeding when needed while periodically introducing recombination through hybrid backcrossing, thereby resolving the contradiction between inbreeding speed and recombination capability.

Inventive Principle:
Principle #19Periodic action

3Adaptability or versatility

If recurrent crossing to hybrid parents is performed, then genetic diversity is maintained, but breeding complexity increases

Engineering Contradiction:
Improvegenetic diversityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding program into distinct phases: initial inbred parent crosses, followed by structured backcrossing generations, and final selection phases. This segmentation of the complex recurrent crossing process into manageable stages reduces overall program complexity while maintaining genetic diversity benefits, as each segment can be independently managed and optimized.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS8581027B2Forward breeding
Publication Date: 2013.11.12 SEMINIS VEGETABLE SEEDS INC
  • US8581027B2 patent drawing
  • US8581027B2 patent drawing

AI summary

A method for plant breeding comprising the steps of: selecting a donor parent possessing a trait of interest; crossing the donor parent to a first hybrid parent; selecting a first generation progeny of the cross; and crossing the first generation progeny with a second hybrid parent to produce a hybrid back cross.