Fragaria Powdery Mildew Resistance DNA Marker Panel

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Solution Overview

Problem

Current DNA marker technologies for powdery mildew resistance in strawberries are insufficient in terms of LOD and contribution ratio, lacking effective markers for evaluating resistance accurately.

Innovation Solution

Development of multiple DNA markers in the genus Fragaria, specifically identifying a nucleic acid region linked to powdery mildew resistance, enabling accurate evaluation and selection of resistant plant lines through linkage analysis and nucleic acid amplification reactions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional DNA marker technologies are used for powdery mildew resistance detection, then the detection process can be performed, but the LOD value and contribution ratio are insufficient, resulting in low measurement precision

Engineering Contradiction:

Improvepowdery mildew resistance evaluation accuracyVSAvoidmarker effectiveness

Core Design Contradiction:

Measurement precisionVSReliability

Solution Approach 1:

The patent divides the detection task into multiple independent marker assays. Instead of relying on a single marker with limited precision, the invention uses a panel of multiple DNA markers (including SSR and SNP markers) that collectively provide comprehensive and accurate powdery mildew resistance evaluation, thereby resolving the contradiction between measurement precision and reliability

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent combines multiple types of DNA markers (SSR markers and SNP markers) into a composite marker system. This composite approach integrates the advantages of different marker types to achieve both high LOD values and high contribution ratios, simultaneously improving measurement precision and reliability for powdery mildew resistance detection

Inventive Principle:

Principle #40Composite materials

2Measurement precision

If field testing is conducted to evaluate powdery mildew resistance, then direct resistance assessment is possible, but the process is time-consuming and costly

Engineering Contradiction:

Improveresistance assessment accuracyVSAvoidselection process duration

Core Design Contradiction:

Measurement precisionVSLoss of time

Solution Approach 1:

The patent performs preliminary DNA marker-based screening to identify plant lines with desired powdery mildew resistance characteristics before conducting actual field testing. This preliminary genetic assessment filters out unsuitable candidates early in the selection process, reducing the number of plants that require time-consuming field tests while maintaining accurate resistance assessment for the selected candidates

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent replaces the mechanical field testing system with a molecular biology-based DNA marker detection system. Instead of physically exposing plants to powdery mildew in field conditions, the invention uses in vitro DNA analysis to predict resistance characteristics, dramatically reducing the time and resources required for resistance assessment while maintaining high accuracy

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS11384395B2Marker associated with powdery mildew resistance in plant of genus <i>Fragaria </i>and use thereof

Publication Date: 2022.07.12 TOYOTA JIDOSHA KK

AI summary

This invention intends to develop many DNA markers for a plant of the genus Fragaria and detect powdery mildew resistance with high precision by using the many DNA markers. The marker associated with powdery mildew resistance in a plant of the genus Fragaria comprises a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 19 in the chromosome of the plant of the genus Fragaria.