Free AIM Immunoassay with SRCR2-Selective Antibodies
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Solution Overview
Problem
Existing methods struggle to accurately measure the amount of free AIM in biological samples due to the interference from complex AIM, necessitating a technique to selectively detect free AIM without complex AIM.
Innovation Solution
Development of an anti-AIM monoclonal antibody that specifically reacts with free AIM by recognizing the SRCR2 domain, allowing for the development of immunoassay methods and kits with high specificity and sensitivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional immunoassay methods are used to detect AIM, then both free AIM and complex AIM are detected, but the measurement precision for free AIM is poor
Solution Approach 1:
The invention segments the detection target into two distinct forms: free AIM and complex AIM. By developing monoclonal antibodies with different specificities (one recognizing only free AIM, another recognizing both free and complex AIM), the assay can differentiate and selectively measure free AIM in the presence of complex AIM, thereby improving measurement precision
Solution Approach 2:
The invention changes the binding parameter of the antibody by selecting monoclonal antibodies with different epitope specificities. The first monoclonal antibody is selected to bind specifically to free AIM, while the second binds to both free and complex AIM. This parameter change in antibody specificity enables the differentiation and accurate measurement of free AIM
2Measurement precision
If methods are developed to selectively detect free AIM, then measurement precision improves, but device complexity increases
Solution Approach 1:
The invention extracts the specific binding capability from the antibody pool by selecting monoclonal antibodies with defined specificities. The first monoclonal antibody is extracted to recognize only free AIM, while the second is extracted to recognize both forms. This extraction of specific binding properties simplifies the assay design compared to using polyclonal antibodies or complex separation methods
Solution Approach 2:
The second monoclonal antibody serves multiple functions: it can detect both free AIM and complex AIM, and when used in combination with the first monoclonal antibody, it enables selective measurement of free AIM through competitive binding or signal differentiation. This multi-functionality reduces the need for additional reagents or complex equipment
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate detection of free AIM in biological samples containing both free and complex AIM, enhancing diagnostic precision.
Implementation Method 1
an anti-AIM monoclonal antibody having a property of reacting with free AIM and not reacting with complex AIM
Data Source
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AI summary
A problem to be solved is to detect only free AIM in a biological sample containing complex AIM and free AIM. The problem can be solved by an immunoassay method for free AIM in a biological sample using an anti-AIM monoclonal antibody having a property of reacting with free AIM and not reacting with complex AIM, wherein the anti-AIM monoclonal antibody is an antibody binding to an epitope in the SRCR2 domain of human AIM.