Freeze-Dried PRP Composition With Primed Platelet Stability

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Solution Overview

Problem

Existing PRP compositions contain a high percentage of activated platelets due to the use of cryopreservatives, limiting their therapeutic applications and requiring immediate use or freeze-drying, and there is a need for compositions with improved storage properties and higher percentages of primed platelets.

Innovation Solution

Developed PRP compositions with at least 40% primed platelets that are not fully activated, prepared without cryopreservatives, and can be freeze-dried for long-term storage, allowing reconstitution to release regenerative molecules upon administration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If cryopreservatives are added to preserve platelets during freezing, then storage stability is improved, but platelet activation increases (80% activated)

Engineering Contradiction:

Improvestorage stabilityVSAvoidplatelet activation state

Core Design Contradiction:

Stability of the object's compositionVSReliability

Solution Approach 1:

The patent changes the chemical composition parameters by eliminating cryopreservatives and instead using specific anticoagulants (citrate-based or phosphate-based) to maintain platelet stability during storage. This parameter change resolves the contradiction by achieving storage stability through a different chemical mechanism that does not induce platelet activation.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent employs a disposable platelet collection and processing system where platelets are collected, processed into PRP, and either used immediately or freeze-dried without cryopreservatives. The system accepts that platelets have limited viability but optimizes the short window of usability by preventing activation during storage through the specified anticoagulant formulation.

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

2Reliability

If platelets are stored immediately or freeze-dried without delay, then platelet activation is reduced, but storage flexibility and long-term availability are limited

Engineering Contradiction:

Improveplatelet activation stateVSAvoidstorage flexibility

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent applies preliminary action by adding specific anticoagulants (citrate or phosphate buffers at defined concentrations) to the platelet-containing plasma before storage or freeze-drying. This preliminary chemical protection allows the platelets to be stored longer and transported without activation, providing storage flexibility while maintaining reliability.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent utilizes phase transition (freeze-drying or lyophilization) to convert liquid PRP into a stable solid form for long-term storage. By combining this phase transition with the specific anticoagulant formulation that prevents activation, the patent achieves both long-term storage flexibility and maintained platelet reliability upon reconstitution.

Inventive Principle:

Principle #36Phase transitions

3Speed

If platelets are fully activated before administration, then immediate hemostatic effect is achieved, but regenerative molecule release occurs prematurely limiting therapeutic applications

Engineering Contradiction:

Improvehemostatic effect speedVSAvoidtherapeutic application range

Core Design Contradiction:

SpeedVSAdaptability or versatility

Solution Approach 1:

The patent applies partial action by using anticoagulants that partially inhibit platelet activation pathways without completely blocking them. This allows platelets to retain their regenerative molecules while maintaining the capacity for rapid activation when needed, achieving both sustained therapeutic potential and adequate hemostatic speed.

Inventive Principle:

Principle #16Partial or excessive action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The compositions maintain a high percentage of primed platelets, ensuring effective therapeutic delivery with enhanced storage stability and regenerative capabilities.

Implementation Method 1

The compositions are freeze-dried for long term storage and do not contain cryoprotectants or cryopreservatives

Methodology Applied

Scientific EffectFreeze-drying: Freeze Drying

Data Source

PatentUS20260061003A1Platelet-rich plasma compositions, preparation, and uses thereof

Publication Date: 2026.03.05 VETSTEM INC

AI summary

Provided are platelet-rich plasma (PRP) compositions, including freeze-dried (lyophilized) and reconstituted compositions containing primed platelets. The compositions are freeze-dried for long term storage and do not contain cryoprotectants or cryopreservatives.