FRET-PAINT Multiplexed Biomarker Detection Kit
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Solution Overview
Problem
Conventional biomarker detection methods, such as ELISA, require large amounts of biological samples and multiple detection kits, imposing physical and economic burdens and limiting early disease diagnosis, especially for simultaneous detection of multiple biomarkers.
Innovation Solution
The method employs FRET-PAINT technology, where detection probes conjugated with docking strands bind to target substances, and separate strands labeled with donor and acceptor fluorescent substances generate fluorescence signals through FRET, allowing for sensitive and multiplexed detection of multiple biomarkers from small sample volumes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional ELISA techniques are used for biomarker detection, then detection sensitivity is maintained at several to tens of pg/ml, but large amounts of biological samples and multiple detection kits are required
Solution Approach 1:
The patent combines multiple detection functions into a single detection kit that can simultaneously detect multiple biomarkers (including cancer markers, cardiac markers, and infectious disease markers) in one test, eliminating the need for multiple separate ELISA kits and large sample volumes
Solution Approach 2:
The detection kit is designed with universal applicability to detect various types of biomarkers using a single platform, allowing one kit to perform multiple detection functions across different disease categories, thereby reducing the quantity of samples and kits needed
2Measurement precision
If multiple separate examinations are performed to detect multiple biomarkers, then each biomarker can be identified individually, but the examination process is repeated multiple times and requires sufficient blood samples
Solution Approach 1:
The patent merges multiple separate biomarker detection examinations into a single multiplexed detection system that can identify multiple biomarkers simultaneously from one blood sample, maintaining diagnostic accuracy while minimizing sample requirements
Solution Approach 2:
The detection system adds a dimension of multiplexing capability, allowing simultaneous detection of multiple biomarkers in parallel within a single examination platform, transforming the sequential detection process into a concurrent one
3Adaptability or versatility
If a large number of detection kits are used for simultaneous biomarker detection, then multiple biomarkers can be detected, but economic burden and difficulty in early diagnosis increase
Solution Approach 1:
The invention creates a universal detection platform that can detect multiple biomarkers across different disease categories (cancer, cardiac, infectious diseases) using a single kit type, eliminating the need for multiple specialized kits and reducing overall system complexity
Solution Approach 2:
The detection kit is segmented into modular components including capture probes, detection probes, and signal generation systems that can work together in various combinations to detect different biomarkers, providing versatility without requiring separate kits for each marker
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly enhances sensitivity, enabling the detection of multiple biomarkers from very small sample amounts, improving early disease diagnosis and reducing the need for extensive sample collection and kit usage.
Implementation Method 1
either the docking strands or the separate strands, or both, are labeled with at least one donor fluorescent substance and at least one acceptor fluorescent substance, and measuring fluorescence signals generated by the FRET between the donor fluorescent substance and the acceptor fluorescent substance
Data Source
AI summary
Provided is a method for detecting target substances. The method includes a) introducing a sample containing target substances onto a substrate, b) allowing detection probes conjugated with docking strands to specifically bind to the target substances, c) introducing one or more separate strands capable of complementary binding to the docking strands into the docking strands, either the docking strands or the separate strands, or both, are labeled with at least one donor fluorescent substance and at least one acceptor fluorescent substance, and d) measuring fluorescence signals generated by the FRET between the donor fluorescent substance and the acceptor fluorescent substance to identify the target substances.