Concave-Eared Frog Peptide Ot-WHP for Cost-Effective Wound Healing
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Solution Overview
Problem
Current wound healing treatments face challenges due to high molecular weights and production costs of growth factors, and the mechanisms of amphibian-derived wound healing peptides are not thoroughly understood, limiting their clinical application.
Innovation Solution
An immunomodulating peptide (Ot-WHP) derived from the concave-eared torrent frog, with a specific amino acid sequence, is used to promote wound healing by inducing chemokines and cytokine production, activating signaling pathways, and enhancing the migration and proliferation of cells, particularly in the presence of macrophages, at a lower molecular weight and cost.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If growth factors (EGF, FGF-2, VEGF, PDGF, KGF-1, GM-CSF, G-CSF) are used to promote wound healing, then wound healing effect is improved, but production cost and molecular weight increase
Solution Approach 1:
The patent extracts and utilizes the active peptide sequence Ot-WHP from the concave-eared torrent frog skin secretion. By identifying and isolating the specific 24-amino acid sequence (SEQ ID NO:1) responsible for wound healing activity, the invention extracts only the essential functional component rather than using entire complex growth factor molecules, thereby reducing production cost while maintaining therapeutic effect
Solution Approach 2:
The patent creates a simplified copy of the natural amphibian peptide Ot-WHP through chemical synthesis or genetic engineering. Instead of producing complex natural growth factors, the invention synthesizes a smaller peptide copy that replicates the key wound healing function at lower cost and with simpler manufacturing processes
2Reliability
If growth factors are used to promote wound healing, then wound healing effect is improved, but molecular weight increases
Solution Approach 1:
The patent segments the complex growth factor molecules into smaller functional units by identifying and utilizing only the essential 24-amino acid Ot-WHP sequence. This segmentation reduces molecular weight from thousands of Daltons (growth factors) to 2666.19 Daltons (Ot-WHP peptide), while preserving the core wound healing functionality through the segmented active sequence
3Ease of manufacture
If amphibian-derived wound healing peptides are used, then production cost is reduced, but mechanism of action is not thoroughly understood
Solution Approach 1:
The patent implements comprehensive feedback mechanisms to elucidate the mechanism of action of Ot-WHP. Through in vitro cell migration assays, in vivo wound healing models, and signaling pathway analysis, the invention continuously gathers data on how Ot-WHP functions, thereby understanding its chemotactic effects on neutrophils and macrophages, its regulation of effector cells, and its interaction with signaling pathways like MAPK and NF-κB
Data Source
AI summary
The present invention relates to an immunomodulating peptide comprising an amino acid sequence as shown in SEQ ID NO:1. The present invention also discloses use of the immunomodulating peptide in the preparation of a drug or skin care product for promoting skin wound healing. The present invention provides a new immunomodulating peptide and use thereof, and discloses the mechanism of action of the immunomodulating peptide in promoting wound healing. The immunomodulating peptide is useful as a wounding healing polypeptide template.