Frozen Reagent Measurement Unit for Biological Sample Analysis

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Solution Overview

Problem

Existing biological sample measurement techniques face challenges in maintaining reagent quality and simplifying operations, particularly when reagents deteriorate at room temperature and require immediate preparation and application near the subject, complicating the measurement process.

Innovation Solution

A measurement unit with a first container housing a frozen mixture of reagents, including a fluorescent indicator, and a second container with a frozen stimulant solution, both designed for easy transport and defrosting near the subject, allowing for simplified operations and maintaining reagent quality by avoiding immediate preparation near the subject.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If reagents are prepared and operations are performed immediately near the subject, then reagent quality is maintained, but operational complexity increases

Engineering Contradiction:
Improvereagent qualityVSAvoidoperational complexity
Core Design Contradiction:
ReliabilityVSEase of operation

Solution Approach 1:

The reagents are pre-mixed and frozen in a container before use. This preliminary preparation allows the reagents to be stored stably without deterioration, while the actual mixing and application occur immediately near the subject when needed, thus maintaining both reagent quality and operational simplicity.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The reagent mixture is frozen to a solid state for stable storage and transport, then thawed immediately before use. This phase transition allows the reagents to maintain quality during storage while enabling immediate application near the subject, resolving the contradiction between maintaining quality and simplifying operations.

Inventive Principle:
Principle #36Phase transitions

2Ease of manufacture

If reagents are stored at room temperature, then storage is simple, but reagent quality deteriorates

Engineering Contradiction:
Improvestorage simplicityVSAvoidreagent quality
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The reagent mixture is frozen to a solid state for storage, which prevents deterioration while maintaining storage simplicity. The container is designed to be easily thawed before use, allowing the reagents to be prepared immediately without complex storage or handling procedures.

Inventive Principle:
Principle #36Phase transitions

Solution Approach 2:

The temperature parameter of the reagent mixture is changed to below freezing point to prevent deterioration. This parameter change allows simple storage while maintaining reagent quality, and the container is designed to facilitate quick return to usable temperature before application.

Inventive Principle:
Principle #35Parameter changes

3Reliability

If reagents are prepared near the subject, then reagent quality is maintained, but the measurement process becomes complicated

Engineering Contradiction:
Improvereagent qualityVSAvoidmeasurement process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The reagents are pre-prepared and frozen in a portable container that can be brought to the subject. This eliminates the need for complex on-site preparation equipment and procedures, simplifying the measurement process while maintaining reagent quality through immediate use after thawing.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The frozen container acts as an intermediary that stores pre-prepared reagents and delivers them to the measurement location. This intermediary device eliminates the need for complex on-site reagent preparation systems, simplifying the overall measurement process while ensuring reagent quality through controlled storage and immediate application.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The solution ensures the quality of the reagents is maintained, simplifies the measurement process, and promotes efficient defrosting and dispersion of the biological sample, enhancing the accuracy and ease of biological sample characterization.

Implementation Method 1

a first solution that is a mixture of a plurality of reagents containing an indicator reacting with a component generated from the biological sample, in which the first solution is housed in the first housing space in a state of being frozen

Methodology Applied
Scientific EffectFreezing: Freezing

Data Source

PatentUS20240361242A1Measurement unit, method for manufacturing measurement unit, and biological sample measurement method
Publication Date: 2024.10.31 HAMAMATSU PHOTONICS KK
  • US20240361242A1 patent drawing
  • US20240361242A1 patent drawing
  • US20240361242A1 patent drawing

AI summary

A measurement unit is a measurement unit used for measuring a characteristic of a biological sample. The measurement unit includes a first container that includes a first housing space, a first opening communicating with the first housing space, and a light transmission region through which light generated in the first housing space is transmitted, and a first solution that is a mixture of a plurality of reagents containing an indicator reacting with a component generated from the biological sample. The first solution is housed in the first housing space in a state of being frozen.