Heterotrophic Microalgal Cultivation Using Fruit Wastewater

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Solution Overview

Problem

Traditional wastewater treatment systems for high BOD wastewater from dried fruit industries and wineries are inefficient and costly, and there is a need for a sustainable and cost-effective method to utilize this wastewater for microalgal cultivation to produce valuable bioproducts like biofuels and lipids.

Innovation Solution

Utilizing wastewater from dried fruit and winery industries as a sugar feedstock for heterotrophic microalgal cultivation, specifically using strains like Botryococcus and Neochloris, under controlled light conditions to enhance growth and product accumulation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional wastewater treatment systems are used to treat high BOD wastewater from dried fruit and winery industries, then BOD levels can be reduced to meet discharge requirements, but the treatment process is inefficient and costly with significant operational complexity

Engineering Contradiction:
ImproveBOD treatment effectivenessVSAvoidtreatment system complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent converts the harmful high-BOD wastewater into a beneficial resource by using it as a sugar feedstock for heterotrophic microalgal cultivation. The microalgae consume the sugar in the wastewater for growth, thereby reducing BOD levels while producing valuable bioproducts like lipids and biofuels. This transforms the waste treatment problem into a value-added production process.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The microalgal system provides self-service by using the wastewater's own sugar content as its carbon source for growth. The microalgae naturally consume the organic matter in the wastewater, reducing BOD levels while generating biomass that can be converted into products. This eliminates the need for external carbon sources and reduces operational complexity.

Inventive Principle:
Principle #25Self-service

2Reliability

If traditional wastewater treatment systems are used, then BOD discharge levels can be controlled, but the operational costs and investment requirements are significantly high

Engineering Contradiction:
ImproveBOD discharge controlVSAvoidtreatment cost
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent transforms the cost center of wastewater treatment into a profit center by producing saleable bioproducts. The microalgae convert wastewater sugars into lipids and other valuable compounds that can be extracted and sold, thereby offsetting or exceeding the operational costs of the treatment process.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent changes the operational parameters from pure treatment mode to production mode. By optimizing conditions for microalgal growth and product accumulation (such as controlling light exposure, nutrients, and harvest timing), the system maximizes bioproduct yield while maintaining BOD reduction, thereby improving the economic viability of the process.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If wastewater is used as sugar feedstock for microalgal cultivation, then valuable bioproducts like biofuels and lipids can be produced with higher bioproductivity, but the system requires careful control of growth conditions to ensure effective treatment and product accumulation

Engineering Contradiction:
ImprovebioproductivityVSAvoidgrowth condition control
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The microalgal system largely self-regulates by responding naturally to the availability of sugar in the wastewater. When sugar is abundant, microalgae grow rapidly and consume the substrate, reducing BOD. This natural response reduces the need for complex external control systems while maintaining high productivity.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent employs parameter changes to optimize both treatment and production. By adjusting parameters such as light intensity, aeration rates, and harvest frequency, the system can shift between maximizing biomass production and maximizing product accumulation, thereby achieving high bioproductivity while maintaining treatment effectiveness.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method provides a cost-effective and environmentally friendly solution for wastewater treatment while enabling the economic production of microalgal products such as biofuels, lipids, and other chemicals, with higher bioproductivity compared to traditional systems.

Implementation Method 1

cultivating a microalgae capable of heterotrophic growth, including: incubating the microalgae under a heterotrophic growth condition for a period of time sufficient to allow the microalgae to grow

Methodology Applied
Scientific EffectHeterotrophic growth: Fermentation

Implementation Method 2

wherein the heterotrophic growth condition further includes light

Methodology Applied
Scientific EffectPhotosynthesis: Photosynthesis

Data Source

PatentUS10479969B2Utilization of wastewater for microalgal cultivation
Publication Date: 2019.11.19 PHYCOIL BIOTECHNOLOGY INTERNATIONAL INC
  • US10479969B2 patent drawing
  • US10479969B2 patent drawing
  • US10479969B2 patent drawing

AI summary

Bioreactors and methods for cultivating microalgae under heterotrophic growth conditions using fruit wastewater as a carbon source are provided herein. The heterotrophic growth conditions include low irradiance of light sufficient to initiate light-activated metabolism, but not photosynthesis. The methods provide a cost effective and environmentally friendly solution for converting wastewater from dried fruit and wine industries into products like biofuels.