FTIR Sensor Surface for Particle Redispersion Detection
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Solution Overview
Problem
Current biosensing methods face challenges in reliably detecting the redispersion of dry label particles into solution, which is crucial for visualizing and quantifying small amounts of specific molecules, as existing techniques lack precision in monitoring this process.
Innovation Solution
The method employs Frustrated Total Internal Reflection (FTIR) to measure the redispersion of dry particles into solution by illuminating a sensor surface with light and detecting changes in reflected light intensity as particles redisperse, allowing for the analysis of both the total amount and rate of redispersion.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If dry particles are used in biosensing, then storage stability and transport convenience are improved, but detection precision and reliability of redispersion monitoring deteriorate
Solution Approach 1:
The patent introduces an intermediary measurement approach by using FTIR to detect the refractive index changes in the liquid medium during particle redispersion, rather than directly observing the particles themselves. This intermediary measurement enables precise monitoring of the redispersion process while maintaining the stability advantages of dry particle storage
Solution Approach 2:
The patent monitors changes in optical parameters (refractive index, light intensity) during the redispersion process. By tracking these parameter changes over time, the system can precisely detect when particles have fully redispersed, thereby achieving high measurement precision without compromising the storage stability benefits of dry particles
2Device complexity
If conventional detection methods are used, then device complexity is reduced, but reliability of redispersion detection deteriorates
Solution Approach 1:
The patent replaces direct mechanical or visual observation methods with optical field-based FTIR detection. This substitution maintains relatively simple device architecture while significantly improving the reliability of redispersion detection through sensitive optical measurements of refractive index changes
3Measurement precision
If FTIR is used to detect redispersion, then measurement precision and reliability are improved, but device complexity and cost increase
Solution Approach 1:
The patent designs the FTIR system to serve multiple functions: it not only detects particle redispersion but can also potentially monitor binding events and other biosensing measurements. This multi-functionality justifies the increased device complexity by providing versatile measurement capabilities within a single integrated platform
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach provides a reliable and precise method for detecting the redispersion of particles, enabling effective monitoring of the binding process and quantification of analytes, enhancing the accuracy of biosensing applications.
Implementation Method 1
the angle between first optical path and sensor surface fulfils the condition of total internal reflection... light illuminating the sensor surface along the first optical path is completely reflected at said sensor surface
Implementation Method 2
If the index of refraction close to said sensor surface is inhomogeneous, e.g., due to the presence of said dry particles, the condition of total internal reflection is - at least partially - violated. This leads to scattering of light at this inhomogeneity
Implementation Method 3
the present invention is based on the idea to use Frustrated Total Internal Reflection (FTIR) to measure the redispersion of dry particles into solution
Data Source
Figure 1~2b
Figure 3a~4b
AI summary
The invention provides a method ofdetecting the redispersion of particles into a solution by means of FTIR or other optical detection methods comprising the following steps: providing a sensor surface with dry particles; illuminating the sensor surface with light along a first optical path and detecting the light reflected by the sensor surface; providing a liquid to a volume in contact with the sensor surface; and detecting the reflected light while the dryparticles redisperse into the liquid. Therein, the angle between first optical path and sensor surface fulfils the condition of total internal reflection. The present invention also provides an FTIR cartridge for use in said method comprising a sensor surface accessible for FTIR detection, said sensor surface comprising at least one binding area, wherein label particles are situated on said surface.