Fungi Fermentation Product Inducing Mesenchymal Epithelial Transition

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

The biological application potential of fungi crops like shiitake mushrooms and black fungus is limited due to their underutilized value, particularly in converting cancer cells into normal cells through a mesenchymal epithelial transition mechanism.

Innovation Solution

A method involving the preparation of a fungi fermentation product containing N-acetylglucosamine, achieved by treating and fermenting specific fungi species such as Lentinula edodes and Cordyceps militaris, which induces mesenchymal-epithelial transitions in cancer cells, converting them into epithelial cells.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If fungi crops are used for human consumption, then they provide nutritional value, but their biological application potential is limited

Engineering Contradiction:

Improvebiological application potentialVSAvoidadded value

Core Design Contradiction:

Adaptability or versatilityVSQuantity of substance

Solution Approach 1:

The patent changes the functional parameters of fungi crops by transforming them through fermentation processes to produce N-acetylglucosamine-containing products with cancer cell conversion capabilities, thereby expanding their biological application potential beyond traditional nutritional use

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent introduces N-acetylglucosamine as an intermediary substance produced during fermentation that serves as the active agent for converting cancer cells into normal cells, enabling the fungi products to achieve therapeutic effects

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If the fungi fermentation product is used to convert cancer cells into normal cells, then therapeutic potential is enhanced, but the complexity of the fermentation process increases

Engineering Contradiction:

Improvetherapeutic potentialVSAvoidfermentation process complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent segments the fermentation process into distinct stages with specific fungi species assigned to different phases, allowing complex biochemical transformations to be managed through controlled sequential operations

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent maintains continuous fermentation processes under controlled conditions to ensure consistent production of N-acetylglucosamine and maintain the therapeutic activity required for cancer cell conversion

Inventive Principle:

Principle #20Continuity of useful action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method effectively transforms human liver cancer cells into normal epithelial cells, enhancing the therapeutic potential of fungi-derived products and increasing their value by demonstrating the induction of mesenchymal-epithelial transitions, as evidenced by shape changes and E-cadherin restoration in Huh7 cells.

Implementation Method 1

the fungi fermentation product of the present invention induces mesenchymal-epithelial transitions (MET), so that Huh7 cells are converted from the mesenchymal cells into the epithelial cells

Methodology Applied

Scientific EffectMesenchymal-epithelial transition:

Implementation Method 2

in turn culturing the Cordyceps fungus under a solid state and a liquid state successively to form a Cordyceps fungus liquid

Methodology Applied

Scientific EffectSolid state culture:

Implementation Method 3

in turn culturing the Cordyceps fungus under a solid state and a liquid state successively to form a Cordyceps fungus liquid

Methodology Applied

Scientific EffectLiquid state culture:

Implementation Method 4

heating and sterilizing the fungi liquid to form a fungi fruiting body or mycelium liquid

Methodology Applied

Scientific EffectHeating: Heating

Implementation Method 5

mixing and culturing the fungi fruiting body or mycelium liquid and the Cordyceps fungus liquid under a proper condition to form the fungi fermentation product containing N-acetylglucosamine

Methodology Applied

Scientific EffectFermentation: Fermentation

Data Source

PatentUS20240050501A1Method for Forming Fungi Fermentation Product Containing N-Acetylglucosamine to Convert Cancer Cells into Normal Cells Through Mesenchymal Epithelial Transition Mechanism

Publication Date: 2024.02.15 YANG KUN LIN

AI summary

The invention discloses a method for forming a fungi fermentation product containing N-acetylglucosamine. The fungi fermentation product containing N-acetylglucosamine can convert cancer cells into normal cells through a mesenchymal epithelial transition mechanism. The method uses fungi as a starting material which together with a Cordyceps-related fungus is fermented to form a fermentation product which induces a mesenchymal epithelial transition to convert cancer cells into normal cells. The fungi cell wall contains chitin. The fermentation liquid is produced by fermentation of Cordyceps with chitin as the substrate. The main product is N-acetylglucosamine confirmed by an HPLC analysis. According to the experimental data, the fermentation product of N-acetylglucosamine converts cancer cells into epithelial cells that contain E-cadherin and has a square shape. Thus, it is proved that the fermented substance converts cancer cells into normal cells through induction of the mesenchymal epithelial transition mechanism.