Filamentous Fungi Iron Chelation Process

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Solution Overview

Problem

Current iron supplements, such as elemental iron and ferric pyrophosphate, have low absorption rates in humans due to their insoluble nature, making them ineffective in combating anemia, especially in developing countries where widespread fortification is needed.

Innovation Solution

A process using filamentous fungi like Aspergillus oryzae to chelate insoluble iron forms from elemental iron powders, ferric pyrophosphate, and other compounds, converting them into bioavailable organic iron through fermentation, resulting in a nutritional supplement with high iron content.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If insoluble iron compounds (elemental iron, ferric pyrophosphate, ferric orthophosphate) are used for food fortification, then stability and non-reactive nature are improved, but absorption rate by humans deteriorates (2-20% absorption rate)

Engineering Contradiction:
ImprovestabilityVSAvoidabsorption rate
Core Design Contradiction:
Stability of the object's compositionVSReliability

Solution Approach 1:

Filamentous fungi act as an intermediary organism that transforms insoluble iron compounds into soluble organic iron forms. The fungi uptake insoluble iron from the culture medium and convert it into metabolizable organic iron within their biomass, creating a bridge between stable insoluble iron and bioavailable soluble iron forms.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The chemical form of iron is changed from insoluble inorganic compounds to soluble organic compounds through biological transformation. This parameter change in solubility and chemical bonding state dramatically improves iron bioavailability while maintaining stability for fortification applications.

Inventive Principle:
Principle #35Parameter changes

2Reliability

If soluble iron forms (ferrous sulfate) are used, then absorption rate is improved, but stability and non-reactive nature deteriorates

Engineering Contradiction:
Improveabsorption rateVSAvoidstability
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The iron is transformed into an organic chelated form within the fungal biomass that combines the best properties of both soluble and stable iron forms. The organic iron compounds produced by fungi exhibit both high solubility for absorption and stability for fortification applications.

Inventive Principle:
Principle #35Parameter changes

3Reliability

If microorganisms are used to chelate iron, then bioavailability is improved, but process complexity increases

Engineering Contradiction:
ImprovebioavailabilityVSAvoidprocess complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The filamentous fungi perform the iron transformation function autonomously through their natural metabolic processes. The organism self-regulates the uptake and conversion of iron without requiring external intervention or complex control systems, simplifying the overall process despite the biological complexity.

Inventive Principle:
Principle #25Self-service

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The process produces a nutritional supplement with elevated organic iron levels, achieving absorption rates comparable to ferrous sulfate, reducing side effects and enhancing bioavailability, suitable for human and animal consumption.

Implementation Method 1

Filamentous fungi have the ability to chelate a high level iron from the soluble inorganic compounds

Methodology Applied
Scientific EffectChelation: Adsorption

Implementation Method 2

culturing filamentous fungi A. oryzae (A. o.) in the culture medium to accumulate iron in the filamentous fungi as metabolizable organic iron

Methodology Applied
Scientific EffectBiological absorption: Absorption (physical)

Data Source

PatentEP3462915B1Process for forming iron enriched nutritional products
Publication Date: 2021.04.14 CURA GLOBAL HEALTH BVI LTD
  • EP3462915B1 patent drawingFigure 1

AI summary

The technology relates to a process for forming a nutritional supplement containing iron by providing a culture medium containing insoluble iron and culturing filamentous fungi in the culture medium to accumulate iron in the filamentous fungi as metabolizable organic iron. The technology also relates to uses of the nutritional supplement as food additive or supplement.