Extracellular Polysaccharide Extraction from Fusarium Solani
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Solution Overview
Problem
There is a need for an efficient method to extract and utilize the extracellular polysaccharides from the Dendrobium officinale endophytic fungus strain, Fusarium solani, which has potential antibacterial properties, due to the endangered status of D. officinale and the limited availability of wild resources.
Innovation Solution
A method involving the activation, culturing, and vacuum filtration of the D. officinale endophytic fungus strain, followed by alcohol precipitation, centrifugation, dialysis, and freeze-drying to obtain a freeze-dried extracellular polysaccharide powder, which is shown to inhibit pathogens such as Escherichia coli, Staphylococcus aureus, Salmonella ssp., and Bacillus subtilis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If wild Dendrobium officinale resources are used for polysaccharide extraction, then the polysaccharide can be obtained, but the wild resources are exhausted and the plant becomes endangered
Solution Approach 1:
The invention extracts the endophytic fungus from the Dendrobium officinale plant and isolates it as a separate culture system. The fungus is then used to produce polysaccharides in controlled fermentation conditions, extracting the valuable metabolic product without depleting the plant population in the wild.
Solution Approach 2:
The invention creates a copy of the polysaccharide production capability by culturing the endophytic fungus in controlled environments. Instead of harvesting from wild plants, the same polysaccharide can be produced by cultivating the isolated fungus under controlled conditions, replicating the natural production process.
2Productivity
If traditional extraction methods are used, then the process is simple, but the extraction efficiency and purity of polysaccharide are limited
Solution Approach 1:
The extraction process is divided into distinct sequential steps: fermentation to produce extracellular polysaccharides, filtration to separate cells from supernatant, concentration to remove excess water, alcohol precipitation to isolate polysaccharides, dialysis to remove small molecules, and freeze-drying to obtain pure product. Each step targets a specific purification requirement.
Solution Approach 2:
Alcohol is used as an intermediary substance to precipitate the polysaccharides from the fermented broth. The alcohol causes the polysaccharides to precipitate out of solution while leaving other soluble components in the supernatant, enabling selective isolation and purification.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The extracted polysaccharide demonstrates significant antibacterial activity against the mentioned pathogens, providing a feasible and scalable process for pathogen inhibition and potentially enhancing host plant interactions.
Implementation Method 1
filtering the fermented liquid under vacuum
Implementation Method 2
concentrated the filtrate under vacuum
Implementation Method 3
dialyzing after dissolving the precipitates in water
Implementation Method 4
freezing and drying the dialysate under vacuum
Data Source
AI summary
The present invention provides a Dendrobium officinale (D. officinale) endophytic fungus strain and an extracellular polysaccharide produced thereby as well as an extraction method and application of the extracellular polysaccharide. The extraction process of the extracellular polysaccharide includes: activating a strain; culturing seed liquid; fermenting and culturing; filtering the fermented liquid under vacuum; concentrated the filtrate under vacuum; conducting alcohol precipitation on the concentrated liquid; precipitating and centrifuging; dialyzing after dissolving the precipitates in water; freezing and drying the dialysate under vacuum; and obtaining the extracellular polysaccharide. The D. officinale endophytic fungus strain belongs to Fusarium solani, and the extracellular polysaccharide produced thereby has inhibitory effects on Escherichia coli, Staphylococcus aureus, Salmonella ssp., and Bacillus subtilis.