Inbred Corn Line G07-NPID2449 CMS Breeding System

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Solution Overview

Problem

The development of inbred corn lines with specific agronomic traits is challenging due to the complexity of genetic systems governing traits like yield, disease resistance, and adaptability to different soil types and environments, making it difficult to produce hybrids that are uniformly adapted across the Corn Belt regions.

Innovation Solution

The introduction of the inbred corn line G07-NPID2449, which combines unique traits and allows for the production of hybrid seeds through targeted trait introduction using methods like marker-assisted breeding and transgenic genes, enabling improved yield, disease resistance, and adaptability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop inbred corn lines with specific agronomic traits, then breeding programs can proceed with conventional approaches, but the complexity of genetic systems makes it difficult to produce hybrids uniformly adapted across different regions

Engineering Contradiction:
ImproveadaptabilityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent introduces a male sterility system as an intermediary mechanism to simplify hybrid seed production. By using cytoplasmic male sterility (CMS) in the seed parent, the need for manual detasseling is eliminated, and cross-pollination is automatically ensured. This intermediary genetic system resolves the contradiction by providing a reliable method for producing uniformly adapted hybrids without complex manual intervention across different growing regions.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent employs parameter changes by introducing specific genetic traits (male sterility, fertility restoration genes) that alter the reproductive parameters of the corn plants. By changing the sterility parameter through cytoplasmic factors and nuclear restorer genes, the system enables controlled cross-pollination and produces hybrids with consistent adaptability across different environments without requiring complex regional-specific breeding programs.

Inventive Principle:
Principle #35Parameter changes

2Ease of manufacture

If CMS (cytoplasmic male sterility) is introduced into inbred lines to eliminate detasseling, then hybrid seed production becomes simpler, but poor agronomic performance traits are introduced causing farmer concern

Engineering Contradiction:
Improvehybrid seed production easeVSAvoidagronomic performance reliability
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent applies the extraction principle by separating the male sterility function from the agronomic performance traits. The CMS trait is introduced only in the seed parent line specifically for the purpose of eliminating detasseling, while the maintainer and restorer lines retain normal agronomic performance. This extraction allows the sterility function to be taken out and applied only where needed, preventing the spread of poor agronomic traits to the final hybrid product.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The breeding program is segmented into three distinct lines with specific functions: the CMS seed parent line (for eliminating detasseling), the maintainer line (for preserving the CMS trait and maintaining agronomic performance), and the restorer line (for restoring fertility and providing good agronomic traits). This segmentation allows each line to be optimized for its specific function, ensuring that poor agronomic performance remains isolated to the seed parent and does not affect the final hybrid's reliability.

Inventive Principle:
Principle #1Segmentation

3Ease of operation

If three different maize lines are required for CMS system (male sterile line, maintainer line, restorer line), then detasseling is eliminated, but the breeding complexity increases requiring breeding of all three types of lines

Engineering Contradiction:
Improvefield operation easeVSAvoidbreeding system complexity
Core Design Contradiction:
Ease of operationVSDevice complexity

Solution Approach 1:

The CMS system enables self-service in hybrid seed production by automatically preventing self-pollination through cytoplasmic male sterility. The seed parent line cannot produce viable pollen, so cross-pollination from the restorer line occurs automatically without requiring manual detasseling or other mechanical interventions. This self-service mechanism eliminates labor-intensive operations while the standardized three-line system provides a repeatable breeding framework that reduces long-term complexity.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS7790968B1Inbred corn line G07-NPID2449
Publication Date: 2010.09.07 SYNGENTA CROP PROTECITON AG

AI summary

Basically, this invention provides for an inbred corn line designated G07-NPID2449, methods for producing a corn plant by crossing plants of the inbred line G07-NPID2449 with plants of another corn plants. The invention relates to the various parts of inbred G07-NPID2449 including culturable cells. This invention also relates to methods for introducing transgenic transgenes into inbred corn line G07-NPID2449 and plants produced by said methods.